Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.
Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.
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DOI：
10.1364/ol.40.002653
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发表时间：
2015-06-01
期刊：
影响因子：
3.6
通讯作者：
Ha T
中科院分区：
文献类型：
--
作者：
Han KY;Ha T
我们描述了一种双色三维受激发射耗尽（3D-STED）显微镜，采用一个单一的激光源，重复频率为80 MHz。利用光子晶体光纤产生与STED脉冲同步的多个激发脉冲，并通过具有高光谱纯度的声光可调谐滤波器选择所需的波长。在不同波长的选择性激发允许同时成像的两个荧光标记在纳米级分辨率的三维。
We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.