A DEVELOPMENTAL SWITCH IN THYMIC LYMPHOCYTE MATURATION POTENTIAL OCCURS AT THE LEVEL OF HEMATOPOIETIC STEM-CELLS
A DEVELOPMENTAL SWITCH IN THYMIC LYMPHOCYTE MATURATION POTENTIAL OCCURS AT THE LEVEL OF HEMATOPOIETIC STEM-CELLS
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DOI：
10.1016/0092-8674(90)90262-d
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发表时间：
1990-09-07
期刊：
影响因子：
64.5
通讯作者：
WEISSMAN, IL
中科院分区：
文献类型：
--
作者：
IKUTA, K;KINA, T;WEISSMAN, IL
从小鼠胎肝分离的造血干细胞（HSC）与成体HSC一样是Thy-1 loLin-Sca-1+。在体外用胎肝HSC再增殖的胎儿胸腺叶中检测到供体来源的V γ 3 + T细胞，但在用成人骨髓HSC再增殖的胸腺叶中未检测到。单克隆胎儿HSC产生胸腺子代，其包括V γ 3+、其它γ。δ +，和α。β + T细胞。在胸腺内注射胎儿或成人HSC的成人胸腺中未检测到V γ 3 + T细胞。这些结果支持以下假设：只有胎儿HSC具有在胎儿胸腺微环境中分化成V γ 3 + T细胞的能力，并且HSC的发育潜能可能在个体发育期间发生变化。
Hematopoietic stem cells (HSCs) isolated from mouse fetal liver, like adult HSCs, are Thy-1lo Lin- Sca-1+. Donor-derived V.gamma.3+ T cells were detected in fetal thymic lobes repopulated in vitro with fetal liver HSCs, but not in those with adult bone marrow HSCs. Single clonogenic fetal HSCs gave rise to thymic progeny that include V.gamma.3+, other .gamma..delta.+, and .alpha..beta.+ T cells. No V.gamma.3+ T cells were detected in adult thymus injected intrathymically with either fetal or adult HSCs. These results support the hypothesis that only fetal HSCs have the capacity to differentiate into V.gamma.3+ T cells in the fetal thymic microenvironment and that the developmental potential of HSCs may change during ontogeny.