Use of magnetically purified Plasmodium falciparum parasites improves the accuracy of erythrocyte invasion assays
Use of magnetically purified Plasmodium falciparum parasites improves the accuracy of erythrocyte invasion assays
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DOI：
10.1016/j.exppara.2010.05.007
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发表时间：
2010-10-01
影响因子：
2.1
通讯作者：
Su, Xin-zhuan
中科院分区：
文献类型：
--
作者：
Bates, Adam H.;Mu, Jianbing;Su, Xin-zhuan
裂殖子侵入红细胞是恶性疟原虫无性循环的关键步骤。通过检测纯化的寄生虫进入具有不同表面受体的红细胞，无论是突变体还是在不同的酶处理下，恶性疟原虫有多种入侵途径，涉及不同的配体-受体相互作用。成功的侵袭试验最关键的一步是分离感染有活力的裂殖体的红细胞。在此，我们应用磁化柱来纯化裂殖体以进行红细胞侵袭试验。与Percoll-山梨醇纯化方法相比，改进的方法在侵袭试验的重复性和可靠性方面有了很大的提高，特别是对于短期的、培养适应的寄生虫分离株。对于不耐受Percoll-山梨醇或对Percoll-山梨醇具有未知敏感性的寄生虫分离株来说，磁提纯方法是一个很好的替代方法。由爱思唯尔公司出版。
Merozoite invasion of erythrocytes is a crucial step for the asexual cycle of Plasmodium falciparum. Multiple invasion pathways, which involve different ligand-receptor interactions, have been identified in P. falciparum by examining the entry of purified parasite into erythrocytes with different surface receptors, either mutant or under different enzyme treatments. The most critical step for a successful invasion assay is the isolation of erythrocytes infected with viable schizonts. Here, we applied a magnetic column to purify the schizonts for the erythrocyte invasion assay. Comparing to Percoll-sorbitol purification method, this modified approach showed great improvement on reproducibility and reliability of invasion assay, particularly for short-term, culture-adapted parasite isolates. The magnetic purification method is an excellent alternative for parasite isolates that do not tolerate or with unknown sensitivity to Percoll-sorbitol exposure. Published by Elsevier Inc.