Memory antibody response from antigen loaded polymer particles and the effect of antigen release kinetics
Memory antibody response from antigen loaded polymer particles and the effect of antigen release kinetics
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DOI：
10.1016/j.biomaterials.2009.05.075
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Panda, Amulya K.
中科院分区：
文献类型：
--
作者：
Kanchan, Vibhu;Katare, Yogesh K.;Panda, Amulya K.
记忆抗体反应是持久免疫的标志。在这项研究中，我们报告了记忆抗体反应的产生，而免疫与单剂量的聚合物颗粒包埋抗原。用明矾和包埋抗原的聚乳酸（PLA）聚合物颗粒（28 μ m）的混合物进行免疫接种，不仅引起持久的初级抗体应答，而且在再次暴露于非常少量的可溶性抗原时也引起非常高水平的记忆抗体滴度。在破伤风类毒素（TT）的情况下，来自基于PLA颗粒的免疫的记忆抗体滴度几乎是从两个剂量的明矾吸附抗原获得的记忆抗体滴度的四倍，并且在更长的时间段内维持在更高的水平。甚至在初次免疫18个月后攻击动物后也检测到记忆抗体应答。在用包埋白喉类毒素（DT）的PLA颗粒免疫的情况下，也观察到类似的增强记忆抗体应答。由聚合物制剂产生的记忆抗体应答是高度抗原特异性的。将具有不同抗原释放曲线的聚合物颗粒用作模型系统以评价抗原对免疫记忆的作用。结果表明，从聚合物颗粒中缓慢和连续释放抗原在从单点免疫引发改善的记忆抗体应答中起关键作用。(C)2009爱思唯尔有限公司保留所有权利。
Memory antibody response is the hallmark of long lasting immunity. In this study, we report the generation of memory antibody response while immunizing with single dose of polymer particle entrapped antigens. Immunization with admixture of alum and polylactide (PLA) polymer particles (28 mu m) entrapping antigens not only elicited long lasting primary antibody response but also very high levels of memory antibody titer upon re-exposure to very small amount of soluble antigen. In the case of tetanus toxoid (TT), the memory antibody titers from PLA particle based immunization were almost four times higher than that achieved from two doses of alum adsorbed antigen and sustained at a higher level for a longer period of time. Memory antibody response was detected even after challenging the animals after 18 months of primary immunization. Similar enhanced memory antibody response was also observed in the case of immunization with PLA particle entrapping diphtheria toxoid (DT). Memory antibody response generated from polymeric formulations was highly antigen specific. Polymer particles with different release profile of antigen were used as a model system to evaluate the role of antigen on immunological memory. The results suggest that slow and continuous release of antigen from polymer particles plays a critical role in eliciting improved memory antibody response from single point immunization. (C) 2009 Elsevier Ltd. All rights reserved.