Enhanced Gold Nanoparticle Based ELISA for a Breast Cancer Biomarker
Enhanced Gold Nanoparticle Based ELISA for a Breast Cancer Biomarker
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DOI：
10.1021/ac902492c
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发表时间：
2010-02-01
影响因子：
7.4
通讯作者：
Merkoci, Arben
中科院分区：
文献类型：
--
作者：
Ambrosi, Adriano;Airo, Federico;Merkoci, Arben
在这项工作中，我们开发了一种光学酶联免疫吸附测定（ELISA）免疫分析的CA 15 -3抗原，一个重要的生物标志物存在于血液样本中，并为后续的乳腺癌的药物治疗有用的分析。金纳米粒子（AuNPs）被用作载体的信号抗体抗CA 15 -3-HRP（辣根过氧化物酶），以实现光信号的放大。在0和60 U/mL之间的范围内，与经典ELISA方法相比，采用AuNPs作为增强剂的测定导致更高的灵敏度和更短的测定时间。将AuNPs应用于市售的ELISA测试可以用于改善重要的免疫分析程序，其中需要更可信的结果。
In this work, we developed an optical enzyme-linked immunosorbent assay (ELISA) immunoassay for the analysis of CA15-3 antigen, an important biomarker present in blood samples and useful for the follow-up of the medical treatment of breast cancer. Gold nanoparticles (AuNPs) were used as carriers of the signaling antibody anti-CA15-3-HRP (horseradish peroxidase) in order to achieve an amplification of the optical signal. In the range between 0 and 60 U/mL, the assay adopting AuNPs as an enhancer resulted in higher sensitivity and shorter assay time when compared to classical ELISA procedures. The application of AuNPs to the commercially available ELISA test can be useful to improve important immunoanalysis procedures where a more confident result is needed.