Label-free fluorescent aptasensor for potassium ion using structure-switching aptamers and berberine
Label-free fluorescent aptasensor for potassium ion using structure-switching aptamers and berberine
复制标题
使用结构转换适体和小檗碱的无标记钾离子荧光适体传感器
DOI：
10.1016/j.saa.2014.10.058
复制
发表时间：
2015-02-05
影响因子：
4.4
通讯作者：
Dong, Chuan
中科院分区：
文献类型：
--
作者：
Guo, Yanqing;Chen, Yanxia;Dong, Chuan
A simple, rapid and label-free fluorescent aptasensor was fabricated for the detection of potassium ion (K+ ion) in aqueous solution using K+ ion-stabilized single stranded DNA (ssDNA) with G-rich sequence as the recognition element and a fluorescent dye, berberine, as the fluorescence probe. In the presence of K+ ion, the G-rich ssDNA is promoted to form the aptamer-target complex with a G-quadruplex conformation, and berberine binding to the G-quadruplex structure results in the enhancement of its fluorescence. The fluorescence intensity of the sensing system displayed a calibration response for K+ ion in the range of 0-1600 mu M with a detection limit of 31 nM (S/N = 3) and a relative standard deviation (RSD) of 0.45%. This label-free fluorescence aptasensor is conveniently and effectively applicable for analysis of K+ ion in blood serum samples with the recovery range of 81.7-105.3%. The assay for detection of potassium ion is easy, economical, robust, and stable in rough conditions. (C) 2014 Elsevier B.V. All rights reserved.
