Cytometry in malaria--a practical replacement for microscopy?
Cytometry in malaria--a practical replacement for microscopy?
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DOI：
10.1002/0471142956.cy1120s65
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发表时间：
2013-07-01
影响因子：
--
通讯作者：
Grimberg, Brian T
中科院分区：
文献类型：
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作者：
Shapiro, Howard M;Apte, Simon H;Grimberg, Brian T
由原生动物疟原虫寄生虫引起的疟疾每年造成约80万人死亡。准确的数字是不确定的，因为推定诊断往往没有确定寄生虫在患者的血液中，无论是通过显微镜，使用吉姆萨的百年历史的染色，或通过更简单的测试，最终依赖于显微镜的质量控制。显微镜本身依赖于训练有素的观察者检测寄生红细胞的细微形态特征的能力，只有少数可能出现在载玻片上。定量和客观的细胞成分，如DNA，RNA，和疟疾色素疟原虫色素的流式细胞术测量，现在在疟疾生物学和药理学的研究是有用的，可以提供更可靠的寄生虫种类和发育阶段的鉴定和更好的检测低密度寄生虫血症比显微镜。同样的测量现在可以在更小、更简单、更便宜的成像细胞仪中实现，可能提供更准确和精确的诊断模式。
Malaria, caused by protozoan Plasmodium parasites, kills ~800,000 people each year. Exact figures are uncertain because presumptive diagnoses are often made without identifying parasites in patients' blood either by microscopy, using Giemsa's century-old stain, or by simpler tests that are ultimately dependent on microscopy for quality control. Microscopy itself relies on trained observers' ability to detect subtle morphological features of parasitized red blood cells, only a few of which may be present on a slide. Quantitative and objective flow cytometric measurements of cellular constituents such as DNA, RNA, and the malaria pigment hemozoin are now useful in research in malaria biology and pharmacology, and can provide more reliable identification of parasite species and developmental stages and better detection of low-density parasitemia than could microscopy. The same measurements can now be implemented in much smaller, simpler, cheaper imaging cytometers, potentially providing a more accurate and precise diagnostic modality.