BACKSCATTERED ELECTRON IMAGING OF SKELETAL TISSUES
BACKSCATTERED ELECTRON IMAGING OF SKELETAL TISSUES
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DOI：
10.1016/0221-8747(83)90016-4
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
JONES, SJ
中科院分区：
文献类型：
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作者：
BOYDE, A;JONES, SJ
研究了固体背散射电子（BSE）探测器在骨骼组织扫描电镜研究中的应用。为了最小化图像中的形貌元素，使用平坦样品和环形检测器配置，其中样品垂直入射到光束和检测器。非常平坦的样品通过金刚石微磨或金刚石抛光塑料包埋组织制备。图像中的密度区分是如此之好，以至于可以对矿化骨内的不同密度相位进行成像。对于未嵌入的松质骨，可以通过地形对比机制将切割表面与自然表面区分开。BSE成像还呈现出对于具有粗糙形貌的未包埋样品的优势，例如软骨中矿化区的无机制剂，其引起常规二次电子成像的严重充电问题。
The use of solid-state back-scattered electron (BSE) detectors in the scanning electron microscopic study of skeletal tissues was investigated. To minimize the topographic element in the image, flat samples and a ring detector configuration with the sample at normal incidence to the beam and the detector are used. Very flat samples are prepared by diamond micromilling or diamond polishing plastic-embedded tissue. Density discrimination in the image is so good that different density phases within mineralized bone can be imaged. For unembedded spongy bone, cut surfaces can be discriminated from natural surfaces by a topographic contrast mechanism. BSE imaging also presents advantages for unembedded samples with rough topography, such as inorganic preparations of the mineralization zone in cartilage, which give rise to severe charging problems with conventional secondary electron imaging.