Seroprevalence of Autoantibodies against Brain Antigens in Health and Disease
Seroprevalence of Autoantibodies against Brain Antigens in Health and Disease
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DOI：
10.1002/ana.24189
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发表时间：
2014-07-01
影响因子：
11.2
通讯作者：
Ehrenreich, Hannelore
中科院分区：
文献类型：
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作者：
Dahm, Liane;Ott, Christoph;Ehrenreich, Hannelore
目的：我们先前报道了在健康和神经精神病受试者（N = 2，817）中N-甲基-D-天冬氨酸受体亚单位-NR 1（NMDAR 1）自身抗体（AB）的血清阳性率出乎意料的高（接近10%）。这一发现挑战了血清AB与脑部疾病之间明确的因果关系。为了测试对于先前与病理条件相关的其他脑抗原导向的AB是否会获得类似的结果，我们系统地筛选了4，236个个体的血清样品。（n = 1，703）与神经精神病受试者（精神分裂症、情感障碍、中风、帕金森病、肌萎缩性侧索硬化、人格障碍;总数n = 2，533）。对于间接免疫荧光分析的基础上，我们使用生物芯片镶嵌的冷冻脑切片（大鼠，猴）和转染HEK 293细胞表达各自的重组靶antigens.Results：血清阳性率的所有筛选AB是可比的健康和生病的个人。然而，它们中没有一个达到NMDAR 1 AB的丰度（同样类似于10%;免疫球蛋白[IG] G类似于1%）。观察到AB抗两性霉素（2.0%）、ARHGAP 26（1.3%）、CASPR 2（0.9%）、MOG（0.8%）、GAD 65（0.5%）、Ma 2（0.5%）、Yo（0.4%）和Ma 1（0.4%）的频率较高，组间滴度和IG类分布相似。所有其他AB均在
Objective: We previously reported an unexpectedly high seroprevalence (similar to 10%) of N-methyl-D-aspartate-receptor subunit-NR1 (NMDAR1) autoantibodies (AB) in healthy and neuropsychiatrically ill subjects (N = 2,817). This finding challenges an unambiguous causal relationship of serum AB with brain disease. To test whether similar results would be obtained for other brain antigen-directed AB previously connected with pathological conditions, we systematically screened serum samples of 4,236 individuals.Methods: Serum samples of healthy (n = 1,703) versus neuropsychiatrically ill subjects (schizophrenia, affective disorders, stroke, Parkinson disease, amyotrophic lateral sclerosis, personality disorder; total n = 2,533) were tested. For analysis based on indirect immunofluorescence, we used biochip mosaics of frozen brain sections (rat, monkey) and transfected HEK293 cells expressing respective recombinant target antigens.Results: Seroprevalence of all screened AB was comparable in healthy and ill individuals. None of them, however, reached the abundance of NMDAR1 AB (again similar to 10%; immunoglobulin [Ig] G similar to 1%). Appreciable frequency was noted for AB against amphiphysin (2.0%), ARHGAP26 (1.3%), CASPR2 (0.9%), MOG (0.8%), GAD65 (0.5%), Ma2 (0.5%), Yo (0.4%), and Ma1 (0.4%), with titers and Ig class distribution similar among groups. All other AB were found in