Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates
Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates
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DOI：
10.1007/s10327-017-0752-5
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发表时间：
2017
影响因子：
1.2
通讯作者：
T. Hasegawa;Atsushi Okabe;Y. Kato;A. Ooshiro;H. Kawaide;M. Natsume
中科院分区：
文献类型：
--
作者：
T. Hasegawa;Atsushi Okabe;Y. Kato;A. Ooshiro;H. Kawaide;M. Natsume
采用生物测定法对番茄根系分泌物中的青枯菌化学引诱物进行了分析。番茄品种Oogata-fukuju根系分泌物的活性炭吸附组分具有化学引诱活性。茄属菌株MAFF 730138。活性成分，纯化使用Sep-Pak C18柱，活性炭柱，二醇改性硅胶，和NH 2改性硅胶，是一种新的疏水性引诱剂。最终纯化的级分在二醇改性的硅胶HPLC分析中产生单峰。
Tomato root exudates were analyzed using a bioassay to detect the chemoattractant forRalstonia solanacearum. An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity forR. solanacearumstrain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH2-modified silica gel, is a new hydrophobic attractant. The final purified fraction produced a single peak in a diol-modified silica gel HPLC analysis.