HPLC quantitation of a very hydrophilic 3-hydroxypyridin-4-one chelator using a simple separation procedure and the baseline file subtraction method.
HPLC quantitation of a very hydrophilic 3-hydroxypyridin-4-one chelator using a simple separation procedure and the baseline file subtraction method.
复制标题
使用简单的分离程序和基线文件扣除方法对非常亲水的 3-羟基吡啶-4-酮螯合剂进行 HPLC 定量。
DOI：
10.1093/chromsci/34.1.52
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发表时间：
1996
影响因子：
1.3
通讯作者：
Yokel,RA
中科院分区：
文献类型：
--
作者：
Skinner,TL;Meurer,KA;Yokel,RA
缺乏从血液中分离非常亲水的3-羟基吡啶-4-酮（HP）的方法，阻碍了其药代动力学的测定。本研究的目的是开发一种方法来定量这些化合物。采用简单的样品制备方法结合高效液相色谱法定量血浆中的1-[乙-1-醇]-2-甲基-3-羟基吡啶-4-酮（一种非常亲水的HP）。血浆蛋白用三氯乙酸沉淀。基线文件减法用于在存在通过研究方法无法从HP分离的干扰色谱峰的情况下提高该HP的分离度。该方法用于测定该HP在家兔体内的药代动力学。药代动力学结果的精密度与通过已发表方法分离的7种亲脂性HP所得结果相当或更好。新方法略有修改，并用于研究该HP在大鼠中的药代动力学，精密度与通过已发表方法测定的两种亲脂性HP所得结果相当。当其他方法无法从共洗脱干扰物质中充分分离亲水性分析物时，基线文件减法非常有用。
The lack of a method to isolate very hydrophilic 3-hydroxypyridin-4-ones (HPs) from blood has prevented determination of their pharmacokinetics. The objective of this study is to develop a method to quantitate these compounds. A simple sample preparation method coupled with high-performance liquid chromatography is used to quantitate 1-[ethan-1-ol]-2-methyl-3-hydroxypyridin-4-one, a very hydrophilic HP, in plasma. Plasma proteins are precipitated by trichloroacetic acid. The baseline file subtraction method is used to improve the resolution of this HP in the presence of interfering chromatographic peaks that could not be resolved from the HP by the methods investigated. The method is used to determine the pharmacokinetics of this HP in rabbits. The precision of the pharmacokinetic results is comparable or better than the results obtained from seven more lipophilic HPs that were separated by a published method. The new method is slightly modified and used in a study of the pharmacokinetics of this HP in the rat, and precision is comparable with results obtained with two more lipophilic HPs determined by the published method. Baseline file subtraction is useful when other methods cannot be used to adequately resolve a hydrophilic analyte from coeluting interfering substances.