Ellagic acid, a natural polyphenolic compound, induces apoptosis and potentiates retinoic acid-induced differentiation of human leukemia HL-60 cells
Ellagic acid, a natural polyphenolic compound, induces apoptosis and potentiates retinoic acid-induced differentiation of human leukemia HL-60 cells
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DOI：
10.1007/s12185-010-0627-4
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发表时间：
2010-07-01
影响因子：
2.1
通讯作者：
Okabe-Kado, Junko
中科院分区：
文献类型：
--
作者：
Hagiwara, Yuki;Kasukabe, Takashi;Okabe-Kado, Junko
全反式维甲酸（ATRA）是用于急性早幼粒细胞白血病分化治疗的标准药物。为了加强这种治疗，我们研究了鞣花酸（EA），一种天然多酚化合物，具有抗增殖和抗氧化特性，对HL-60急性髓性白血病细胞的生长和分化的影响。电针可诱导细胞凋亡，此作用可被泛caspase抑制剂Z-VAD-FMK阻断。EA激活caspase-3通路，增强ATRA诱导的髓系分化标志物（CD 11b、MRP-14蛋白、粒细胞形态）的表达。这些结果表明，EA是一种有效的凋亡诱导剂，也有效地增强ATRA诱导的HL-60细胞的髓样分化。
All-trans retinoic acid (ATRA) is a standard drug used for differentiation therapy in acute promyelocytic leukemia. To potentiate this therapy, we examined the effect of ellagic acid (EA), a natural polyphenolic compound with antiproliferative and antioxidant properties, on the growth and differentiation of HL-60 acute myeloid leukemia cells. EA was found to induce apoptosis, which was blocked by pan-caspase inhibitor, Z-VAD-FMK. EA activated the caspase-3 pathway and enhanced the expressions of myeloid differentiation markers (CD11b, MRP-14 protein, granulocytic morphology) induced by ATRA treatment. These results indicate that EA is a potent apoptosis inducer and also effectively potentiates ATRA-induced myeloid differentiation of HL-60 cells.