Distribution Patterns of the Radiolarian Nuclei and Symbionts Using DAPI-Fluorescence
Distribution Patterns of the Radiolarian Nuclei and Symbionts Using DAPI-Fluorescence
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发表时间：
2009
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通讯作者：
N. Suzuki;K. Ogane;Y. Aita;Mariko Kato;S. Sakai;T. Kurihara;A. Matsuoka;S. Ohtsuka;Akio Go;Kazumitsu Nakaguchi;Shuhei Yamaguchi;Takashi Takahashi;A. Tuji
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作者：
N. Suzuki;K. Ogane;Y. Aita;Mariko Kato;S. Sakai;T. Kurihara;A. Matsuoka;S. Ohtsuka;Akio Go;Kazumitsu Nakaguchi;Shuhei Yamaguchi;Takashi Takahashi;A. Tuji
本研究首次报道了DAPI的成功应用。（6-二氨基- 2-苯基吲哚）染色技术对6种放射虫目（棘虫目、胶虫目、纳塞拉目、Spumellaria和Taxopodia， Entactinaria除外）中的5种22科进行了鉴定。在DAPI染色后的荧光显微镜下，棘目目6种、胶体目3种、棘目3种、纳塞尔目18种、Taxopod目1种发出蓝光。这些结果和现有资料表明，棘虫属和两个胶蝇科（胶蝇科和胶蝇科）是多核的，而纳塞拉属、Spumellaria、Taxopo- dia和Thalassosphaeridae是单核的。放射菌核的形状、位置和数量不仅在科水平上存在差异，而且在较低的分类水平上也存在差异。即使在一些物种中，如角螺旋体，细胞核也可以从头部移动到囊内
This study is the first report on the successful application of the DAPI (4! ,6-diamidino- 2-phenylindole) staining technique to 22 families of five of the six radiolarian orders (Acantharia, Collodaria, Nassellaria, Spumellaria, and Taxopodia, excluding Entactinaria). A total of six Acan- tharian species, three Collodarian species, three Spumellarian species, 18 Nassellarian species, and one Taxopod species emitted blue light under epifluorescence microscopy after DAPI staining. These results and existing data indicated that Acantharia and two collodarian families (Col- losphaeridae and Sphaerozoidae) are multi-nucleated, whereas Nassellaria, Spumellaria, Taxopo- dia, and Thalassosphaeridae form a single-nucleus group. The shape, location, and number of nuclei in Radiolaria were variable not only at the family level but also at lower taxonomic levels. Even within species such as Spirocyrtis scalaris, nuclei can move from the cephalis to intracapsu-