QUANTITATION OF RNA USING THE POLYMERASE CHAIN-REACTION
QUANTITATION OF RNA USING THE POLYMERASE CHAIN-REACTION
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DOI：
10.1016/0168-9525(93)90137-7
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发表时间：
1993-11-01
影响因子：
11.4
通讯作者：
ENGEL, JD
中科院分区：
文献类型：
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作者：
FOLEY, KP;LEONARD, MW;ENGEL, JD
顺序使用逆转录酶和聚合酶链反应（RT-PCR）可以快速和敏感地检测特定的rna。然而，RT-PCR的最大优势是其卓越的灵敏度，这也限制了它在定量应用中的实用性，因为在扩增过程中，样品之间反应条件的微小变化的影响会被大大放大。最近开发的几种技术绕过了这个问题，允许使用RT-PCR准确定量RNA。
Sequential use of reverse transcriptase and the polymerase chain reaction (RT-PCR) permits rapid and sensitive detection of specific RNAs. However, the greatest advantage of RT-PCR, its remarkable sensitivity, has also limited its usefulness in quantitative applications, since the effects of minor variations in reaction conditions from sample to sample are greatly magnified during the amplification process. Several recently developed techniques circumvent this problem, allowing accurate quantitation of RNA using RT-PCR.