Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2
Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2
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DOI：
10.1002/cm.20146
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发表时间：
2006-10-01
影响因子：
--
通讯作者：
Inaba, Kazuo
中科院分区：
文献类型：
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作者：
Hozumi, Akiko;Satouh, Yuhkoh;Inaba, Kazuo
利用蛋白质组学和免疫化学技术，我们已经确定了轻链和中间链（IC）的外臂动力蛋白从海鞘海鞘精子轴丝。玻璃海鞘外臂动力蛋白含有六条轻链（LC），包括富含亮氨酸的重复蛋白、Tctex 1-和Tctex 2-相关蛋白、一种类似于果蝇路障的蛋白和两种与衣原体LC 8相关的组分。在玻璃海鞘外核动力蛋白中不含硫氧还蛋白结构域。在玻璃海鞘的五个IC中，有三个是海胆动力蛋白的直系同源物：两个是WD重复蛋白，第三个是后生动物精子鞭毛所特有的，包含硫氧还蛋白和核苷二磷酸激酶模块。其余两个玻璃海鞘IC具有广泛的卷曲螺旋结构，并显示序列相似性外臂动力蛋白对接复合物蛋白2（DC 2），首先在衣原体鞭毛中发现。我们最近发现了第三种具有卷曲螺旋结构的DC 2样蛋白，Ci-Axp 66.0，它也以亚化学计量的量与玻璃海鞘外臂动力蛋白相关。此外，Oda 5 p，一个额外的复杂的组件所需的装配外ann动力蛋白在Chlamyclomonas鞭毛，也组与这个家庭的DC 2样蛋白。因此，外臂动力蛋白组装到双微管上涉及与DC 2相关的多个卷曲螺旋蛋白。
Using proteomic and immunochemical techniques, we have identified the light and intermediate chains (IC) of outer arm dynein from sperm axonemes of the ascidian Ciona intestinalis. Ciona outer arm dynein contains six light chains (LC) including a leucine-rich repeat protein, Tctex1- and Tctex2-related proteins, a protein similar to Drosophila roadblock and two components related to Chlamydomonas LC8. No LC with thioredoxin domains is included in Ciona outer ann dynein. Among the five ICs in Ciona, three are orthologs of those in sea urchin dynein: two are WD-repeat proteins and the third one, unique to metazoan sperm flagella, contains both thioredoxin and nucleoside diphosphate kinase modules. The remaining two Ciona ICs have extensive coiled coil structure and show sequence similarity to outer arm dynein docking complex protein 2 (DC2) that was first identified in Chlamydomonas flagella. We recently identified a third DC2-like protein with coiled coil structure, Ci-Axp66.0 that is also associated in substoichiometric amounts with Ciona outer arm dynein. In addition, Oda5p, a component of an additional complex required for assembly of outer ann dynein in Chlamyclomonas flagella, also groups with this family of DC2-like proteins. Thus, the assembly of outer arm dynein onto doublet microtubules involves multiple coiled-coil proteins related to DC2.