Expression of TPX2 in salivary gland carcinomas
Expression of TPX2 in salivary gland carcinomas
复制标题
DOI：
10.3892/or_00000227
复制
发表时间：
2009-02-01
期刊：
影响因子：
4.2
通讯作者：
Kamata, Nobuyuki
中科院分区：
文献类型：
--
作者：
Shigeishi, Hideo;Ohta, Kouji;Kamata, Nobuyuki
TPX 2是一种微管相关蛋白，是哺乳动物细胞中着丝粒处微管形成所必需的。本研究旨在探讨TPX 2 mRNA在涎腺癌组织中的表达及其与临床病理因素的关系。应用实时荧光定量逆转录-聚合酶链反应（RT-PCR）检测20例涎腺癌（粘液表皮样癌8例，腺样囊性癌7例，腺泡细胞癌5例）和6例正常颌下腺组织中TPX 2 mRNA的表达。粘液表皮样癌中TPX 2 mRNA的平均表达水平（0.53 +/- 0.51）高于正常下颌下腺（0.047 +/- 0.029）;发现了显著相关性（Mann-Whitney U检验，P=0.0067）。TPX 2在腺泡细胞癌（0.45 ± 0.49）和腺样囊性癌（0.28 ± 0.22）中的平均表达水平也高于正常颌下腺。发现了统计学相关性（Mann-Whitney U检验，分别为P=0.028和P=0.003）。在这项研究中，TPX 2和透明质酸介导的运动受体（RHAMM）的表达之间的相关性进行了研究。TPX和RHAMM的mRNA表达水平之间存在显著相关性（Pearson相关系数，秩检验，P=0.020）。这些结果表明，人TPX 2 mRNA与恶性涎腺肿瘤中细胞增殖增加或异常密切相关。
TPX2 is a microtubule-associated protein and is required for microtubule formation at kinetochores in mammalian cells. The purpose of this study was to clarify the expression of TPX2 mRNA and correlation between TPX2 and clinicopathological factors in salivary gland carcinomas. The expression of TPX2 mRNA was investigated in 20 human salivary gland carcinomas (8 mucoepidermoid carcinomas, 7 adenoid cystic carcinomas, 5 acinic cell carcinomas) and 6 normal submandibular glands using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). The mean expression level of TPX2 mRNA was higher in mucoepidermoid carcinomas (0.53 +/- 0.51) than in normal submandibular glands (0.047 +/- 0.029); a significant association was found (Mann-Whitney U test, P=0.0067). The mean expression levels of TPX2 were also higher in acinic cell carcinomas (0.45 +/- 0.49) and adenoid cystic carcinomas (0.28 +/- 0.22) than in normal submandibular glands. Statistical correlations were found (Mann-Whitney U test, P=0.028 and P=0.003, respectively). Correlation between expression of TPX2 and receptor for hyaluronan-mediated motility (RHAMM) was also investigated in this study. A significant association was found between the mRNA expression levels of TPX and RHAMM (Pearson's correlation coefficient by rank test, P=0.020). These results indicate that human TPX2 mRNA is closely linked to increased or abnormal cell proliferation in malignant salivary gland tumors.