Reaction of acylated homoserine lactone bacterial signaling molecules with oxidized halogen antimicrobials
Reaction of acylated homoserine lactone bacterial signaling molecules with oxidized halogen antimicrobials
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DOI：
10.1128/aem.67.7.3174-3179.2001
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发表时间：
2001-07-01
影响因子：
4.4
通讯作者：
McCoy, WF
中科院分区：
文献类型：
--
作者：
Brochardt, SA;Allain, EJ;McCoy, WF
氧化卤素抗菌剂，如次氯酸和次溴酸，已广泛用于工业系统中的微生物控制。最近的发现表明，酰基化的同丝氨酸内酯细胞间信号分子对铜绿假单胞菌的生物膜形成很重要，这表明生物污垢可以通过干扰细菌细胞间通讯来控制。本研究旨在探讨氧化卤素与酰基高丝氨酸内酯信号分子反应的可能性。含有3-氧基的酰化同丝氨酸内酯与氧化卤素反应迅速，而缺乏3-氧基的酰化同丝氨酸内酯则不发生反应。采用紫色杆菌CV026生物测定法测定了这些反应对酰化高丝氨酸内酯活性的影响。结果表明，暴露于氧化卤素后，3-氧酰基高丝氨酸内酯活性迅速丧失；然而，酰基化的同丝氨酸内酯缺乏3-氧基保留活性。对海带的实验表明，天然卤素过氧化物酶系统能够介导酰基化的同丝氨酸内酯的失活。这说明了一种天然的防御机制，以防止这种海洋藻类表面的生物污染。紫色细菌活性分析表明，尽管生物膜成分的浓度更高，3-氧酰化的高丝氨酸内酯分子和氧化卤素之间的反应仍然发生。这项工作表明，氧化卤素不仅可以通过杀灭机制控制生物膜，还可能通过干扰3-氧酰化高丝氨酸内酯的细胞信号传导来控制生物膜。
Oxidized halogen antimicrobials, such as hypochlorous and hypobromous acids, have been used extensively for microbial control in industrial systems. Recent discoveries have shown that acylated homoserine lactone cell-to-cell signaling molecules are important for biofilm formation in Pseudomonas aeruginosa, suggesting that biofouling can be controlled by interfering with bacterial cell-to-cell communication. This study was conducted to investigate the potential for oxidized halogens to react with acylated homoserine lactone-based signaling molecules. Acylated homoserine lactones containing a 3-oxo group were found to rapidly react with oxidized halogens, while acylated homoserine lactones lacking the 3-oxo functionality did not react. The Chromobacterium violaceum CV026 bioassay was used to determine the effects of such reactions on acylated homoserine lactone activity. The results demonstrated that 3-oxo acyl homoserine lactone activity was rapidly lost upon exposure to oxidized halogens; however, acylated homoserine lactones lacking the 3-oxo group retained activity, Experiments with the marine alga Laminaria digitata demonstrated that natural haloperoxidase systems are capable of mediating the deactivation of acylated homoserine lactones. This mag illustrate a natural defense mechanism to prevent biofouling on the su-face of this marine alga, The Chromobacterium violaceum activity assay illustrates that reactions bem een 3-oxo acylated homoserine lactone molecules and oxidized halogens do occur despite the presence of biofilm components at much greater concentrations. This work suggests that oxidized halogens may control biofilm not only via a cidal mechanism, but also by possibly interfering with 3-oxo acylated homoserine lactone-based cell signaling.