Effects of Nutrients in Substrates of Different Grains on Aflatoxin B1 Production by Aspergillus flavus.
Effects of Nutrients in Substrates of Different Grains on Aflatoxin B1 Production by Aspergillus flavus.
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DOI：
10.1155/2016/7232858
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发表时间：
2016
影响因子：
--
通讯作者：
Qi D
中科院分区：
文献类型：
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作者：
Liu J;Sun L;Zhang N;Zhang J;Guo J;Li C;Rajput SA;Qi D
为了更好地了解影响黄曲霉毒素B1（AFB 1）积累的潜在因素，研究了不同谷物中AFB 1积累的差异。测定了脱脂基质的营养成分和含量，并根据基质的营养成分，研究了淀粉、可溶性糖、氨基酸和微量元素对黄曲霉毒素B1（AFB 1）产生和菌丝生长的影响。这些结果证实了从研磨的底物中去除脂质显著降低了黄曲霉产生黄曲霉毒素B1的底物的潜力。麦芽糖、葡萄糖、蔗糖、精氨酸、谷氨酸、天冬氨酸和锌显著诱导黄曲霉毒素B1的产生，高达1.7- 26.6倍。而水苏糖对A.黄曲霉的生长量高于其他营养素。因此，本研究表明，结合谷物的营养成分，除了脂肪，蔗糖，水苏糖，谷氨酸和锌可能发挥关键作用，在不同的粮食差异感染A。黄的特别是，我们发现谷物中的两种新营养素（精氨酸和水苏糖）显着刺激AFB 1和A的产生。黄曲霉生长。研究结果为抗真菌方法提供了新的概念，以保护食品和动物饲料免受黄曲霉毒素B1污染。
The current study was to better understand the potential factors affecting aflatoxin B1 (AFB1) accumulation varies between different grains. The nutrient composition and contents of defatted substrates were determined; additionally, according to the nutrient content of the substrates, the effects of starch, soluble sugars, amino acids, and trace elements on AFB1 production and mycelial growth in Czapek-Dox medium were examined. These results verified that removal of lipids from ground substrates significantly reduced the substrate's potential for AFB1 production by Aspergillus flavus. Maltose, glucose, sucrose, arginine, glutamic acid, aspartic acid, and zinc significantly induced AFB1 production up to 1.7- to 26.6-fold. And stachyose more significantly promoted A. flavus growth than the other nutrients. Thus, this study demonstrated that, combined with the nutrients content of grains, in addition to lipids, sucrose, stachyose, glutamic acid, and zinc might play key roles in various grains that are differentially infected by A. flavus. Particularly, two new nutrients (arginine and stachyose) of the grains we found significantly stimulate AFB1 production and A. flavus growth, respectively. The results provide new concepts for antifungal methods to protect food and animal feed from AFB1 contamination.