Microbiology of the subglacial Lake Vostok: first results of borehole-frozen lake water analysis and prospects for searching for lake inhabitants
Microbiology of the subglacial Lake Vostok: first results of borehole-frozen lake water analysis and prospects for searching for lake inhabitants
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DOI：
10.1098/rsta.2014.0292
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发表时间：
2016-01-28
期刊：
影响因子：
5
通讯作者：
Bulat, Sergey A.
中科院分区：
文献类型：
--
作者：
Bulat, Sergey A.
本文研究了埋藏在 4 公里厚的南极冰盖下的冰下沃斯托克湖中是否可能存在微生物生命的问题。它代表了对第一次进入湖泊时获得的唯一可用的冻结湖水样本的分析结果，以及随后对钻孔内冻结的水进行重新取芯的结果。为了进行比较，本研究纳入了早期吸积冰分子微生物学研究获得的结果，重点关注嗜热菌和未知的细菌系统型。首次从可能的清洁水采样的角度描述了两个东方湖渗透点。最后，介绍了目前对沃斯托克湖冰冻水样的研究结果，重点是发现了另一种未知细菌系统型w123-10，与上述在沃斯托克积冰中检测到的未知系统型AF532061有远缘关系，两者均成功通过了所有可能的污染控制。使用洁净室设施和建立污染物库被认为是研究沃斯托克湖微生物的先决条件。沃斯托克湖水体中似乎可能存在尚未记录的微生物生命。总之，寻找湖泊居民的前景是为了尽可能干净地对湖水进行采样，以确保进一步的结果是可靠的。
This article examines the question of the possible existence of microbial life inhabiting the subglacial Lake Vostok buried beneath a 4 km thick Antarctic ice sheet. It represents the results of analysis of the only available frozen lake water samples obtained upon the first lake entry and subsequent re-coring the water frozen within the borehole. For comparison, results obtained by earlier molecular microbiological studies of accretion ice are included in this study, with the focus on thermophiles and an unknown bacterial phylotype. A description of two Lake Vostok penetrations is presented for the first time from the point of view of possible clean water sampling. Finally, the results of current studies of Lake Vostok frozen water samples are presented, with the focus on the discovery of another unknown bacterial phylotype w123-10 distantly related to the above-mentioned unknown phylotype AF532061 detected in Vostok accretion ice, both successfully passing all possible controls for contamination. The use of clean-room facilities and the establishment of a contaminant library are considered to be prerequisites for research on microorganisms from Lake Vostok. It seems that not yet recorded microbial life could exist within the Lake Vostok water body. In conclusion, the prospects for searching for lake inhabitants are expressed with the intention to sample the lake water as cleanly as possible in order to make sure that further results will be robust.