The 46 kDa mannose-6-phosphate receptor contains a signal for basolateral sorting within the 19 juxtamembrane cytosolic residues
The 46 kDa mannose-6-phosphate receptor contains a signal for basolateral sorting within the 19 juxtamembrane cytosolic residues
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DOI：
10.1042/bj3270811
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发表时间：
1997-11-01
影响因子：
4.1
通讯作者：
vonFigura, K
中科院分区：
文献类型：
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作者：
Bresciani, R;Denzer, K;vonFigura, K
46 kDa甘露糖-6-磷酸受体（MPR 46）的胞质结构域包含一个信号，该信号介导受体和报告蛋白向Madin-Darby犬肾细胞基底外侧表面结构域的分选。67个胞质残基的逐步截断表明，19个近膜残基足以进行基底侧分选。丙氨酸/甘氨酸扫描诱变鉴定出Glu-11和Ala-17是残基7和19之间的关键残基。Glu-11对于受体胞质尾部的三个内化信号之一也至关重要[Denzer， Weber, Hille-Rehfeld, von Figura and Pohlmann (1997) Biochem]。[j].科学通报，1999,19(4):444 - 444。基侧分类和内化的信号虽然重叠，但依赖于不同的残基。mpr46的基底外侧分选信号不同于酪氨酸或二苯甲酸基基底外侧分选信号，也缺乏与少数不属于这两类的其他基底外侧信号的相似性。
The cytosolic domain of the 46 kDa mannose-6-phosphate receptor (MPR 46) contains a signal that mediates sorting of the receptor and of a reporter protein to the basolateral surface domain of Madin-Darby canine kidney cells. Progressive truncation, of the 67 cytosolic residues indicated that the 19 juxtamembrane residues are sufficient for basolateral sorting. Alanine/glycine-scanning mutagenesis identified Glu-11 and Ala-17 as the critical residues between residues 7 and 19. Glu-11 is also of critical importance for the one of the three internalization signals in the cytosolic tail of the receptor [Denzer, Weber, Hille-Rehfeld, von Figura and Pohlmann (1997) Biochem. J. 326, 497-505]. Although overlapping, the signals for basolateral sorting and internalization depend on different residues. The basolateral sorting signal of MPR 46 is distinct from tyrosine- or dileucine-based basolateral sorting signals and also lacks similarity to the few other basolateral signals that do not fall into these two classes.