Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone
Long-term hydrocephalus alters the cytoarchitecture of the adult subventricular zone
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DOI：
10.1016/j.expneurol.2014.05.011
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发表时间：
2014-11-01
影响因子：
5.3
通讯作者：
Gonzalez-Perez, Oscar
中科院分区：
文献类型：
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作者：
Campos-Ordonez, Tania;Herranz-Perez, Vicente;Gonzalez-Perez, Oscar
脑积水可继发于脑脊液的产生、流动和/或吸收障碍。脑积水的实验模型，特别是亚急性和慢性脑积水。是少数和有限的。脑积水对脑室下区的影响尚不清楚。本研究的目的是分析长期梗阻性脑积水对脑室下区（侧脑室的神经源性生态位）的影响。我们提出了一种通过阻塞小鼠脑内西尔维乌斯输水管来诱导脑积水的新方法，从而模拟人类输水管狭窄。采用免疫荧光和透射电镜观察120日龄大鼠心室扩张程度和室下区细胞组成，每组18只。在成年患者（年龄在bb0 ~ 18岁）中，通过有输水导管狭窄和无输水导管狭窄患者的磁共振图像分析心室下带、胼胝体和内囊的大小（每组25例）。与非脑积水小鼠相比，60天脑积水小鼠免疫荧光显示Ki67 +和双皮质素+细胞数量减少，电镜分析显示脑室下区神经祖细胞和神经母细胞数量减少。值得注意的是，在脑积水小鼠的脑室下区周围也观察到许多与心室管腔和血管接触的细胞外基质结构（裂片）。在人类中，导水管狭窄患者的室下带、胼胝体和内囊的宽度明显小于无导水管狭窄的年龄和性别匹配的患者。综上所述，幕上脑积水降低了神经祖细胞的增殖率，改变了脑室下区的细胞结构和细胞外基质化合物。在人类中，这种相似的过程减少了脑室下生态位以及胼胝体和内囊的宽度。(C) 2014爱思唯尔公司版权所有。
Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus. are few and limited. and the effects of hydrocephalus on the subventricular zone are unclear. The aim of this study was to analyze the effects of long-term obstructive hydrocephalus on the subventricular zone, which is the neurogenic niche lining the lateral ventricles. We developed a new method to induce hydrocephalus by obstructing the aqueduct of Sylvius in the mouse brain, thus simulating aqueductal stenosis in humans. In 120-day-old rodents (n = 18 per group), the degree of ventricular dilatation and cellular composition of the subventricular zone were studied by immunofluorescence and transmission electron microscopy. In adult patients (age > 18 years), the sizes of the subventricular zone, corpus callosum, and internal capsule were analyzed by magnetic resonance images obtained from patients with and without aqueductal stenosis (n = 25 per group). Mice with 60-day hydrocephalus had a reduced number of Ki67 + and doublecortin + cells on immunofluorescence, as well as decreased number of neural progenitors and neuroblasts in the subventricular zone on electron microscopy analysis as compared to non-hydrocephalic mice. Remarkably, a number of extracellular matrix structures (fractones) contacting the ventricular lumen and blood vessels were also observed around the subventricular zone in mice with hydrocephalus. In humans, the widths of the subventricular zone, corpus callosum, and internal capsule in patients with aqueductal stenosis were significantly smaller than age and gender-matched patients without aqueductal stenosis. In summary, supratentorial hydrocephalus reduces the proliferation rate of neural progenitors and modifies the cytoarchitecture and extracellular matrix compounds of the subventricular zone. In humans, this similar process reduces the subventricular niche as well as the width of corpus callosum and internal capsule. (C) 2014 Elsevier Inc. All rights reserved.