Glutathione S-transferase M1 polymorphism and lung cancer risk in African-Americans
Glutathione S-transferase M1 polymorphism and lung cancer risk in African-Americans
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DOI：
10.1093/carcin/21.11.1971
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发表时间：
2000-11-01
期刊：
影响因子：
4.7
通讯作者：
Brandt-Rauf, PW
中科院分区：
文献类型：
--
作者：
Ford, JG;Li, YL;Brandt-Rauf, PW
谷胱甘肽S转移酶M1可以解毒许多致癌物质，包括香烟烟雾中的多环芳烃。虽然已经发表了一些关于GSTM1基因多态与肺癌之间的关联的研究，但这种关联在非裔美国人中受到的关注有限。我们进行了一项病例对照研究，以调查GSTM1基因多态性在非裔美国人肺癌发生中的作用。用聚合酶链式反应(PCR)检测117例肺癌患者和120例正常对照的GSTM1基因，用Logistic回归分析GSTM1基因纯合性缺失与肺癌发生的优势比(OR)和95%可信区间(CI)。117例患者中37例(31.6%)和120例对照中24例(20.0%)存在GSTM1缺失，调整年龄、性别和吸烟因素后，OR值为2.10(95%可信区间1.07~4.11)。鳞癌的相关系数(OR 2.98，95%CI为1.09-8.19)高于腺癌(OR 1.95，95%CI为0.81-4.66)。我们观察到，在重度吸烟者中，大于或等于30包年的GSTM1缺失基因与肺癌有更强的相关性(OR 4.35，95%CI 1.16-16.23)。这种关联在鳞癌中也被发现(OR6.26，95%CI1.31~29.91)，在结合吸烟与GSTM1基因多态性的分析中，吸烟者与野生型非吸烟者相比有更高的风险(OR8.19，95%CI2.35~28.62)，并且随着吸烟年限的增加，危险性增加(趋势P=0.0001)。我们的结果表明，GSTM1基因多态性在非裔美国人肺癌的发生发展中起作用，并改变了与吸烟相关的肺癌的风险。
Glutathione S-transferase M1 (GSTM1) can detoxify many carcinogens, including polycyclic aromatic hydrocarbons such as those from cigarette smoke. Though a number of studies have been published about the association between GSTM1 polymorphism and lung cancer, this association has received limited attention in the African-American population. We conducted a case-control study to investigate the role of GSTM1 polymorphism in the development of lung cancer in African-Americans. Specimens of DNA from 117 lung cancer cases and 120 controls were assayed for detection of GSTM1 genotype by polymerase chain reaction (PCR), The odds ratios (ORs) and 95% confidence intervals (CIs) for lung cancer associated with homozygous deletion of the GSTM1 gene and other risk factors were estimated by logistic regression. Thirty-seven of the 117 cases (31.6%) and 24 of the 120 controls (20.0%) had the GSTM1 null genotype; the OR was 2.10 (95% CI 1.07-4.11) after adjustment for age, gender and smoking. The association was higher for squamous cell carcinoma (OR 2.98, 95% CI 1.09-8.19) than for adenocarccnonma (OR 1.95, 95% CI 0.81-4.66). We observed a stronger association between GSTM1 null genotype and lung cancer among heavy smokers with greater than or equal to 30 pack-years (OR 4.35, 95% CI 1.16-16.23). This association was also found in squamous cell carcinoma (OR 6.26, 95% CI 1.31-29.91), In the analysis combining GSTM1 polymorphism and smoking, smokers with the null genotype had high risk (OR 8.19, 95% CI 2.35-28.62) compared with non-smokers with the wild-type genotype, and the risk increased with smoking cigarette pack-years (P = 0.0001 for trend). Our results suggest that GSTM1 polymorphism plays a role in the development of lung cancer and modifies the risk for smoking-related lung cancer in African-Americans.