Chitin perception in plasmodesmata identifies subcellular, context-specific immune signalling in plants
Chitin perception in plasmodesmata identifies subcellular, context-specific immune signalling in plants
复制标题
DOI：
10.1101/611582
复制
发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Cécilia Cheval;M. Johnston;Sebastian Samwald;Xiaokun Liu;Annalisa Bellandi;A. Breakspear;Y. Kadota;C. Zipfel;C. Faulkner
中科院分区：
文献类型：
--
作者：
Cécilia Cheval;M. Johnston;Sebastian Samwald;Xiaokun Liu;Annalisa Bellandi;A. Breakspear;Y. Kadota;C. Zipfel;C. Faulkner
排列在胞间连丝上的质膜（PM）具有独特的蛋白质和脂质组成，支持细胞之间这些连接的特异性调节。胞间连丝PM可以整合不同于细胞PM的细胞外信号，但目前尚不清楚这种特异性是如何建立的，也不知道单一刺激如何在相邻的膜结构域中触发独立的信号级联。在这里，我们已经使用真菌激发子几丁质研究信号的整合和响应在胞间连丝PM。我们发现，胞间连丝PM采用由LysM受体LYM 2和LYK 4组成的受体复合物，它们分别改变它们的位置和相互作用以响应几丁质。下游，信号通过NADPH氧化酶的特异性磷酸化特征和导致胞间连丝闭合的局部胼胝质合成来传递。这证明了胞间连丝PM部署胞间连丝特异性组分和PM共同组分的差异激活以独立地整合免疫信号。
The plasma membrane (PM) that lines plasmodesmata has a distinct protein and lipid composition, underpinning specific regulation of these connections between cells. The plasmodesmal PM can integrate extracellular signals differently from the cellular PM, but it is not known how this specificity is established or how a single stimulus can trigger independent signalling cascades in neighbouring membrane domains. Here we have used the fungal elicitor chitin to investigate signal integration and responses at the plasmodesmal PM. We found that the plasmodesmal PM employs a receptor complex composed of the LysM receptors LYM2 and LYK4 which respectively change their location and interactions in response to chitin. Downstream, signalling is transmitted via a specific phosphorylation signature of an NADPH oxidase and localised callose synthesis that causes plasmodesmata closure. This demonstrates the plasmodesmal PM deploys both plasmodesmata-specific components and differential activation of PM-common components to independently integrate an immune signal.