A stringent requirement for Plk1 T210 phosphorylation during K-fiber assembly and chromosome congression.
A stringent requirement for Plk1 T210 phosphorylation during K-fiber assembly and chromosome congression.
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DOI：
10.1007/s00412-012-0375-8
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发表时间：
2012-12
期刊：
影响因子：
1.6
通讯作者：
Jallepalli PV
中科院分区：
文献类型：
--
作者：
Paschal CR;Maciejowski J;Jallepalli PV
Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase’s activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1’s kinase activity in vitro, their effects on cell-cycle regulation in vivo remain controversial. Using gene targeting, we replaced the native PLK1 locus in human cells with either PLK1T210A or PLK1T210D, in both dominant and recessive settings. In contrast to previous reports, PLK1T210D did not accelerate cells prematurely into mitosis, nor could it fulfill the kinase’s essential role in chromosome congression. The latter was traced to an unexpected defect in Plk1-dependent phosphorylation of BubR1, a key mediator of stable kinetochore-microtubule attachment. Using chemical genetics to bypass this defect, we found that Plk1T210D is nonetheless able to induce equatorial RhoA zones and cleavage furrows during mitotic exit. Collectively, our data indicate that K-fibers are sensitive to even subtle perturbations in T210 phosphorylation, and caution against relying on Plk1T210D as an in vivo surrogate for the natively activated kinase.
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影响因子：
9.2
作者：
Loncarek, Jadranka;Hergert, Polla;Khodjakov, Alexey
通讯作者：
Khodjakov, Alexey
DOI：
10.1083/jcb.200309035
发表时间：
2004-01-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lindon C;Pines J
通讯作者：
Pines J
DOI：
10.1073/pnas.0701140104
发表时间：
2007-03-13
影响因子：
11.1
作者：
Burkard, Mark E.;Randall, Catherine L.;Jallepalli, Prasad V.
通讯作者：
Jallepalli, Prasad V.
影响因子：
10.5
作者：
Elowe, Sabine;Huemmer, Stefan;Nigg, Erich A.
通讯作者：
Nigg, Erich A.
影响因子：
11.4
作者：
Li, Hongchang;Liu, X. Shawn;Liu, Xiaoqi
通讯作者：
Liu, Xiaoqi