Comparative proteomics analyses for 139A and ME7 scrapie infected mice brains in the middle and terminal stages
Comparative proteomics analyses for 139A and ME7 scrapie infected mice brains in the middle and terminal stages
复制标题
139A和ME7痒病感染中期和末期小鼠大脑的比较蛋白质组学分析
DOI：
10.1002/prca.201600113
复制
发表时间：
2017-01-01
影响因子：
2
通讯作者：
Dong, Xiao-Ping
中科院分区：
文献类型：
--
作者：
Shi, Qi;Chen, Li-Na;Dong, Xiao-Ping
目的分析羊瘙痒病139A株和ME7株感染中晚期小鼠大脑皮层蛋白质组学特征，实验设计采用Western Blot和免疫组化方法分析中晚期小鼠的病理变化.采用iTRAQ技术和多维LC、MS技术对不同发育阶段小鼠的蛋白质组进行分析。本研究还证实了两株羊瘙痒病感染小鼠终末期CNS组织中蛋白表达相似，但中期和终末期样品中蛋白表达明显不同，结论本研究为进一步探讨不同基因表达水平下肿瘤细胞生物学功能的异常提供了线索时间点的朊病毒感染和寻找潜在的治疗和诊断朊病毒疾病的生物标志物。
PurposeTo analyze the proteomics patterns in the cortex regions of scrapie strains 139A- and ME7-infected mice collected in the middle and terminal stages.Experimental designWestern Blot and immunohistochemistry methods are used to analyze the pathological changes in mice collected in the middle and terminal stages. The technique of iTRAQ and multidimensional LC and MS are used to analyze the proteomics patterns of mice in different stages.ResultsIn total, 2891 with 95% confidence interval are identified. The study here also demonstrates a similar protein expressions in the CNS tissues of two scrapie strains infected mice at the terminal stages, but markedly different one between the middle and terminal samples, not only in the numbers of differentially expressed proteins and involved gene ontologies and pathways but also in the relevant functional constitutions.ConclusionsIt may provide useful clue in exploring the abnormalities of biological functions at different time points of prion infections and in searching for potential therapeutic and diagnostic biomarkers for prion diseases.