Meiotically and mitotically stable inheritance of DNA hypomethylation induced by ddm1 mutation of Arabidopsis thaliana.
Meiotically and mitotically stable inheritance of DNA hypomethylation induced by ddm1 mutation of Arabidopsis thaliana.
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发表时间：
1999-02
期刊：
影响因子：
3.3
通讯作者：
T. Kakutani;Kyoko Munakata;E. Richards;H. Hirochika
中科院分区：
文献类型：
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作者：
T. Kakutani;Kyoko Munakata;E. Richards;H. Hirochika
与哺乳动物的表观遗传现象相反，基因表达的重置通常发生在每一代中，植物基因的表观遗传状态通常稳定地通过世代传递。拟南芥突变ddm 1导致基因组5-甲基胞嘧啶水平降低70%。我们以前已经表明，ddm 1突变的结果在一个积累的各种发育异常，通过缓慢诱导其他基因座的遗传变化。每一个检查ddm 1诱导的发育异常是稳定的传输，即使从增强ddm 1突变分离。在这里，遗传的DNA低甲基化ddm 1诱导的异交后代进行了检查，通过HPLC和Southern分析。结果表明，（i）DDM 1基因功能在配子体阶段是不必要的，（ii）ddm 1突变是完全隐性的，（iii）由ddm 1突变低甲基化的序列的再甲基化是非常缓慢或不存在，即使在野生型DDM 1背景。DNA甲基化状态的稳定传递可能与ddm 1诱导的发育异常的减数分裂遗传率有关。
In contrast to mammalian epigenetic phenomena, where resetting of gene expression generally occurs in each generation, epigenetic states of plant genes are often stably transmitted through generations. The Arabidopsis mutation ddm1 causes a 70% reduction in genomic 5-methylcytosine level. We have previously shown that the ddm1 mutation results in an accumulation of a variety of developmental abnormalities by slowly inducing heritable changes in other loci. Each of the examined ddm1-induced developmental abnormalities is stably transmitted even when segregated from the potentiating ddm1 mutation. Here, the inheritance of DNA hypomethylation induced by ddm1 was examined in outcross progeny by HPLC and Southern analyses. The results indicate that (i) DDM1 gene function is not necessary during the gametophyte stage, (ii) ddm1 mutation is completely recessive, and (iii) remethylation of sequences hypomethylated by the ddm1 mutation is extremely slow or nonexistent even in wild-type DDM1 backgrounds. The stable transmission of DNA methylation status may be related to the meiotic heritability of the ddm1-induced developmental abnormalities.