H-1 NOE STUDIES ON DICOPPER(II) DICOBALT(II) SUPEROXIDE-DISMUTASE
H-1 NOE STUDIES ON DICOPPER(II) DICOBALT(II) SUPEROXIDE-DISMUTASE
复制标题
DOI：
10.1021/ic00325a023
复制
发表时间：
1989-12-27
影响因子：
4.6
通讯作者：
TURANO, P
中科院分区：
文献类型：
--
作者：
BANCI, L;BERTINI, I;TURANO, P
由于两种金属离子之间的磁耦合，在铜锌超氧化物歧化酶中用钴(II)取代锌(II)产生适合NMR研究的衍生物。进行 NOE 测量是为了将它们与质子间距离联系起来。在铜域中，一对质子之间的距离小于 3.5 µ，已取得成功。每个组氨酸的三个信号已在坚实的基础上分配，并讨论了阴离子与铜相互作用的含义。
Substitution ofzinc (II) with cobalt (II) in copper zinc superoxide dismutase gives rise to a derivative suitable for· NMR investigation owing to the magnetic coupling between the two metal ions. NOE measurements have been performed in order to relate them to interproton distances. Success has been obtainedwith pairs of protons less than 3.5 Á apart one from the other in the copper domain. The three signals for each histidine have been assigned on a firm basis and the implications for the interaction of anions with copper discussed.