Stem cell antigen-1 expression in the pulmonary vascular endothelium
Stem cell antigen-1 expression in the pulmonary vascular endothelium
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DOI：
10.1152/ajplung.00415.2002
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发表时间：
2003-06-01
影响因子：
4.9
通讯作者：
Fine, A
中科院分区：
文献类型：
--
作者：
Kotton, DN;Summer, RS;Fine, A
虽然细胞表面蛋白干细胞抗原-1（Sca-1）的功能尚未确定，但该分子的表达是骨髓来源的造血干细胞群的特征。然而，Sca-1的表达不限于造血组织。通过RT-PCR和Western分析，我们发现Sca-1在成年小鼠肺组织中有表达。Sca-1免疫组化显示肺大、小动、静脉和肺泡毛细血管内皮表面呈线性染色模式。肺内皮中Sca-1的表达通过肺切片上的双荧光显微镜检查和消化的肺组织的荧光激活细胞分选分析来证实;这些方法中的每一种都显示与内皮标志物血小板/内皮细胞粘附分子-1共定位。在肾脏中，Sca-1表达也在大血管中观察到，但与肺相反，在毛细血管中未观察到。总之，我们的数据表明，Sca-1的表达有助于确定整个肺血管内皮细胞的表面表型。
Although the function of the cell surface protein stem cell antigen-1 (Sca-1) has not been identified, expression of this molecule is a characteristic of bone marrow-derived hematopoietic stem cell populations. Expression of Sca-1, however, is not restricted to hematopoietic tissue. By RT-PCR and Western analysis, we found that Sca-1 is expressed in the adult mouse lung. Sca-1 immunohistochemistry revealed a linear staining pattern on the endothelial surface of large and small pulmonary arteries and veins and alveolar capillaries. Expression of Sca-1 in the pulmonary endothelium was confirmed by dual fluorescent microscopy on lung sections and by fluorescence- activated cell sorting analysis of digested lung tissue; each of these methods showed colocalization with the endothelial marker platelet/endothelial cell adhesion molecule-1. In the kidney, Sca-1 expression was also noted in large vessels, but, in contrast to the lung, was not observed in capillaries. Overall, our data indicate that Sca-1 expression helps define the surface phenotype of endothelial cells throughout the pulmonary vasculature.