FUNCTIONAL-CHARACTERIZATION OF AN ANTIGEN INVOLVED IN AN EARLY STEP OF T-CELL ACTIVATION
FUNCTIONAL-CHARACTERIZATION OF AN ANTIGEN INVOLVED IN AN EARLY STEP OF T-CELL ACTIVATION
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DOI：
10.1073/pnas.84.12.4205
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发表时间：
1987-06-01
影响因子：
11.1
通讯作者：
BARGELLESI, A
中科院分区：
文献类型：
--
作者：
COSULICH, ME;RUBARTELLI, A;BARGELLESI, A
一种活化抗原，由单克隆抗体MLR3鉴定，被描述为存在于活化的T淋巴细胞和胸腺细胞上，但不存在于静止的T淋巴细胞上。活化t细胞系放射标记膜的免疫沉淀表明，mlr3结合分子的分子大小为28-34 kDa。免疫荧光分析显示，在T淋巴细胞和胸腺细胞中，MLR3抗原的出现是一个早期事件，早于白细胞介素2受体的出现。添加MLR3单克隆抗体可抑制静止T细胞对OKT3-Sepharose和白细胞介素1或辅助细胞的增殖反应，但不抑制白细胞介素2依赖性的增殖。在白细胞介素1依赖性人胸腺细胞增殖试验中也获得了类似的结果。此外，用纯化的白细胞介素1培养MLR3阳性细胞时，未观察到MLR3表面抗原的表达。因此，MLR3单克隆抗体似乎可以识别一种抗原，这种抗原参与了与白细胞介素1依赖性功能相关的T细胞激活的早期步骤，并作用于T淋巴细胞和胸腺细胞。
An activation antigen, identified by the monoclonal antibody MLR3, is described that is present on activated T lymphocytes and thymocytes but not on resting T lymphocytes. Immunoprecipitation of radiolabeled membranes from an activated T-cell line showed that the MLR3-binding molecule has a molecular size of 28-34 kDa. Immunofluorescence analysis showed that the appearance of the MLR3 antigen is an early event and precedes that of the interleukin 2 receptor both in T lymphocytes and thymocytes. The proliferation response of resting T cells to OKT3-Sepharose and interleukin 1 or accessory cells, but not the interleukin 2-dependent proliferation, was inhibited by the addition of MLR3 monoclonal antibodies. Similar results were also obtained in an interleukin 1-dependent human thymocyte proliferation assay. In addition when MLR3-positive cells were cultured with purified interleukin 1, MLR3 surfact antigen expression was not observed. Thus MLR3 monoclonal antibody appears to recognize an antigen involved in an early step of T-cell activation related to interleukin 1-dependent functions and on both T lymphocytes and thymocytes.