Desulfurobacterium thermolithotrophum gen. nov., sp. nov., a novel autotrophic, sulphur-reducing bacterium isolated from a deep-sea hydrothermal vent
Desulfurobacterium thermolithotrophum gen. nov., sp. nov., a novel autotrophic, sulphur-reducing bacterium isolated from a deep-sea hydrothermal vent
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DOI：
10.1099/00207713-48-3-701
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发表时间：
1998-07-01
期刊：
影响因子：
--
通讯作者：
Jeanthon, C
中科院分区：
文献类型：
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作者：
L'Haridon, S;Cilia, V;Jeanthon, C
从大西洋中脊采集的深海热液烟囱样品中分离出一种嗜热、厌氧、严格自养的硫还原菌，命名为BSA（T）（T =模式菌株）。革兰氏阴性细胞单个或成对出现，呈高度活动的小杆状。未观察到孢子。生长的温度范围为40至75摄氏度，最佳温度为70摄氏度。在70摄氏度下生长的pH范围为4.4至7.5，最佳温度约为6.0。生长的海盐浓度范围为15-70 g l（-1），最佳浓度为35 g l（-1）。元素硫、硫代硫酸盐和亚硫酸盐被还原成硫化氢。硫酸盐和胱氨酸未减少。基因组DNA的G+C含量为35mol%。16 SrRNA基因系统发育分析表明，该菌株属于细菌属（Bacteria），与水生菌目（Aquificales）和嗜热菌目（Thermotogales）形成了一个分支。因此，建议将该分离物描述为新属的新种，脱硫杆菌新属，模式种为嗜热石生脱硫杆菌Desulfurobacteriumthermolithotrophumsp. nov。模式菌株为BSA（T）（= DSM 11699（T））。
A thermophilic, anaerobic, strictly autotrophic, sulphur-reducing bacterium, designated BSA(T) (T = type strain), was isolated from a deep-sea hydrothermal chimney sample collected at the mid-Atlantic ridge. Gram-negative cells occurred singly or in pairs as small highly motile rods. Spores were not observed. The temperature range for growth was 40 to 75 degrees C, with an optimum at 70 degrees C, The ph range for growth at 70 degrees C was from 4.4 to 7.5, with an optimum around 6.0. The sea salt concentration range for growth was 15-70 g l(-1) with an optimum at 35 g l(-1). Elemental sulphur, thiosulphate and sulphite were reduced to hydrogen sulphide. Sulphate and cystine were not reduced. The G+C content of the genomic DMA was 35 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the strain was a member of the domain Bacteria and formed a branch that was almost equidistant from members of the orders Aquificales and Thermotogales, The new organism possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus; therefore, it is proposed that this isolate should be described as a member of a novel species of a new genus, Desulfurobacterium gen. nov., of which Desulfurobacterium thermolithotrophum sp. nov, is the type species. The type strain is BSA(T) (= DSM 11699(T)).