Human endothelial cell growth and coagulant function varies with respect to interfacial properties of polymeric substrates.
Human endothelial cell growth and coagulant function varies with respect to interfacial properties of polymeric substrates.
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DOI：
10.1002/(sici)1097-4636(199602)30:2
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发表时间：
1996-02
期刊：
影响因子：
--
通讯作者：
K. Kottke-Marchant;A. Veenstra;R. Marchant
中科院分区：
文献类型：
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作者：
K. Kottke-Marchant;A. Veenstra;R. Marchant
研究了人主动脉内皮细胞（HAECs）在一系列从疏水到亲水的聚合物表面上生长时的体外凝血功能。聚合物界面材料由六甲基二硅氮烷、γ-丁内酯和N-乙烯基-2-吡咯烷酮通过射频等离子体聚合制备，并沉积在组织培养Permanox上。三种血浆聚合物无细胞毒性。当预涂有纤连蛋白（FN），HAECs在所有四个聚合物表面是相似的细胞增殖和凝血功能。没有FN预涂层，细胞增殖和扩展增加表面亲水性。组织型纤溶酶原激活物的正常生产增加，在早期孵育时间的聚合物的亲水性增加，组织纤溶酶原激活物/纤溶酶原激活物抑制剂-1的比例。相比之下，在亲水性更强的表面上，标准化的血管性血友病因子释放减少。因此，内皮细胞生长和一些凝血/纤溶功能都随着基质亲水性的增加而改善。
The in vitro coagulant function of human aortic endothelial cells (HAECs) was investigated when grown on a series of polymer surfaces that ranged from hydrophobic to hydrophilic. The polymer interface materials were prepared by radiofrequency plasma polymerization from hexamethyl-disilazane, gamma-butyrolactone, and N-vinyl-2-pyrrolidone and deposited onto tissue culture Permanox. The three plasma polymers were noncytotoxic. When precoated with fibronectin (FN), HAECs on all four polymer surfaces were similar with respect to cell proliferation and coagulant function. Without FN precoating, cell proliferation and spreading increased with increasing surface hydrophilicity. Normalized production of tissue-type plasminogen activator increased with increasing hydrophilicity of the polymers during early incubation times, as did tissue plasminogen activator/plasminogen activator inhibitor-1 ratios. In comparison, normalized von Willebrand factor release decreased on the more hydrophilic surfaces. Thus, both endothelial cell growth and some coagulant/fibrinolytic functions are improved with increasing substrate hydrophilicity.