Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography
Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography
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DOI：
10.1016/j.ophtha.2005.05.023
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发表时间：
2005-10-01
期刊：
影响因子：
13.7
通讯作者：
Duker, JS
中科院分区：
文献类型：
--
作者：
Wojtkowski, M;Srinivasan, V;Duker, JS
目的：展示高速、超高分辨率的三维光学相干层析成像(3D OCT)和视网膜成像的新方案。方法：采用宽带光源的超高分辨率OCT与目前商用的标准10微米分辨率OCT相比，轴向分辨率接近2微米。使用光谱/傅里叶域检测的高速OCT可以显著提高成像速度。使用高速超高分辨率OCT对正常人进行三维OCT视网膜成像。使用密集的栅格扫描图案获取黄斑和视盘的三维OCT数据。展示了新的处理和显示方法，用于生成虚拟OCT眼底图像、任意方向的横断面OCT图像、视网膜、神经纤维层和其他视网膜内层厚度的定量图以及视神经头地形图参数。结果：三维OCT成像使新的成像协议能够改善视网膜微结构的可视化和映射。可以直接从3D OCT数据生成OCT眼底图像，从而实现了横断面OCT图像和厚度图与眼底特征的精确和可重复配准。可以从3D OCT数据生成具有任意方向的光学相干断层成像图像，例如乳突周围扫描。显示了视网膜总厚度和神经纤维层、光感受器层和其他视网膜内层的厚度图。测量视神经头的形态和视盘参数也是可能的。三维OCT可以进行类似于标准仪器的测量，包括StratusOCT、GDX、HRT和RTA。结论：使用高速超高分辨率OCT可以进行三维OCT成像。三维OCT提供了视网膜微结构的全面可视化和映射。高数据采集速度使高密度数据集在视网膜上具有大量的横向位置，从而降低了漏诊局灶性病变的可能性。除了提供图像信息，如OCT横断面图像、OCT眼底图像和3D渲染，还可以定量测量和绘制视网膜内层厚度和视盘的地形图特征。我们希望3D OCT成像可以帮助阐明与视网膜疾病相关的结构变化，以及改善对疾病进展和治疗反应的早期诊断和监测。
Purpose: To demonstrate high-speed, ultrahigh-resolution, 3-dimensional optical coherence tomography (3D OCT) and new protocols for retinal imaging.Methods: Ultrahigh-resolution OCT using broadband light sources achieves axial image resolutions of similar to 2 mu m compared with standard 10-mu m-resolution OCT current commercial instruments. High-speed OCT using spectral/Fourier domain detection enables dramatic increases in imaging speeds. Three-dimensional OCT retinal imaging is performed in normal human subjects using high-speed ultrahigh-resolution OCT. Three-dimensional OCT data of the macula and optic disc are acquired using a dense raster scan pattern. New processing and display methods for generating virtual OCT fundus images; cross-sectional OCT images with arbitrary orientations; quantitative maps of retinal, nerve fiber layer, and other intraretinal layer thicknesses; and optic nerve head topographic parameters are demonstrated.Results: Three-dimensional OCT imaging enables new imaging protocols that improve visualization and mapping of retinal microstructure. An OCT funclus image can be generated directly from the 3D OCT data, which enables precise and repeatable registration of cross-sectional OCT images and thickness maps with fundus features. Optical coherence tomography images with arbitrary orientations, such as circumpapillary scans, can be generated from 3D OCT data. Mapping of total retinal thickness and thicknesses of the nerve fiber layer, photoreceptor layer, and other intraretinal layers is demonstrated. Measurement of optic nerve head topography and disc parameters is also possible. Three-dimensional OCT enables measurements that are similar to those of standard instruments, including the StratusOCT, GDx, HRT, and RTA.Conclusion: Three-dimensional OCT imaging can be performed using high-speed ultrahigh-resolution OCT. Three-dimensional OCT provides comprehensive visualization and mapping of retinal microstructures. The high data acquisition speeds enable high-density data sets with large numbers of transverse positions on the retina, which reduces the possibility of missing focal pathologies. In addition to providing image information such as OCT cross-sectional images, OCT funclus images, and 3D rendering, quantitative measurement and mapping of intraretinal layer thickness and topographic features of the optic disc are possible. We hope that 3D OCT imaging may help to elucidate the structural changes associated with retinal disease as well as improve early diagnosis and monitoring of disease progression and response to treatment.