Biosensors for detecting estrogen-like molecules and protein biomarkers.
Biosensors for detecting estrogen-like molecules and protein biomarkers.
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用于检测雌激素样分子和蛋白质生物标志物的生物传感器。
DOI：
10.1007/978-0-387-74911-2_35
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发表时间：
2008
影响因子：
--
通讯作者：
Erb,JudithL
中科院分区：
文献类型：
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作者：
Wittliff,JamesL;Andres,SarahA;Kruer,TraciL;Kerr2nd,DAlan;Smolenkova,IrinaA;Erb,JudithL
将新型基于渐逝的生物传感器（Endotect™，ThreeFold Sensors，Inc.）以荧光染料标记的重组人雌激素受体-α（rhERα）和hERβ为探针，采用激光光纤技术，建立了一种新的人雌激素受体荧光探针体系。开发了一种三层方法来评估雌激素受体复合物形成的各个步骤及其后续活性，用于仪器校准，以检测生物样本、水和土壤中的雌激素模拟物。使用这种方法，确定某些已知雌激素模拟物的结合亲和力和活性，以用作校准分子。结果表明，rhERα和rhERβ可作为一种特异性较强的雌激素模拟物的探针。此外，该生物传感器用于检测人体组织提取物中的DNA结合蛋白的应用进行了演示。后来的研究表明，生物传感器可用作评估肿瘤标志物蛋白的临床实验室工具。
A novel evanescent-based biosensor (Endotect™, ThreeFold Sensors, Inc.) was developed with laser-based fiber optics using fluorescent dyelabeled recombinant human estrogen receptor-α (rhERα) and hERβ as probes. A three-tiered approach evaluating various steps in the formation of the estrogen- receptor complex and its subsequent activity was developed for instrument calibration to detect estrogen mimics in biological samples, water and soil. Using this approach, binding affinities and activities of certain known estrogen mimics were determined for their use as calibrator molecules. Results indicated rhERα and rhERβ may be employed as probes to distinguish estrogen mimics with a broad range of affinities. In addition, application of the biosensor for detecting DNA-binding proteins in human tissue extracts was demonstrated. The later studies suggest the biosensor may be used as a clinical laboratory tool for assessing tumor marker proteins.