Long-term sampling of viable airborne viruses
Long-term sampling of viable airborne viruses
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DOI：
10.1080/02786820500297012
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发表时间：
2005-09-01
影响因子：
5.2
通讯作者：
Grinshpun, SA
中科院分区：
文献类型：
--
作者：
Agranovski, IE;Safatov, AS;Grinshpun, SA
一种新的生物气溶胶采样技术，利用收集液中的鼓泡过程，最近已经开发出来，并发现空气中的细菌和真菌孢子的长期个人采样是可行的，因为它保持了高的物理收集效率和高微生物回收率的强大和压力敏感的微生物。进一步的测试表明，新技术也有可能收集可行的空气传播病毒，特别是当用于短期采样的强大菌株。由于短期采样在评估生物气溶胶污染环境中的个人暴露方面的应用有限，因此开展了本研究，以调查“起泡器”用于长期监测空气中存活病毒的可行性。用Collison雾化器将含有牛痘病毒粒子（模拟天花病毒，天花的病原体）的液滴雾化到400升的测试室中，在不同的时间段内，通过三个相同的原型个人采样器在液体介质中收集液滴。从1到6小时。使用基于荧光的方法并通过计数每毫升液体中的空斑形成单位来测量采样器的收集液和雾化器的初始悬浮液中的病毒含量。测定采样后病毒的相对回收率。结果表明，“鼓泡”技术具有随时间一致的收集效率，并且能够保持牛痘的活力至少6小时，回收率损失约10%。这些数据表明，新技术具有很好的潜力，可用于测量个人长期暴露于强大的空气传播病毒。
A novel bioaerosol sampling technique, which utilizes the bubbling process in the collection fluid, has recently been developed and found feasible for a long-term personal sampling of airborne bacteria and fungal spores as it maintained high physical collection efficiency and high microbial recovery rate for robust and stress-sensitive microorganisms. Further tests have shown that the new technique also has potential to collect viable airborne viruses, particularly when utilized for a short-term sampling of robust strains. As the short-term sampling has a limited application for assessing personal exposure in bioaerosol-contaminated environments, the present study was undertaken to investigate the feasibility of the "bubbler" for a long-term monitoring of viable airborne viruses. Liquid droplets containing Vaccinia virions ( that simulate Variola, a causative agent of smallpox) were aerosolized with a Collison nebulizer into a 400-liter test chamber, from which the droplets were collected by three identical prototype personal samplers in the liquid medium during different time periods ranging from 1 to 6 hours. The viral content was measured in the collection fluid of the sampler and in the initial suspension of the nebulizer using the fluorescence-based method and by enumerating plaque-forming units per milliliter of the fluids. The relative recovery of viruses after the sampling act was determined. The results show that the "bubbling" technique has consistent collection efficiency over time and is capable of maintaining the viability of Vaccinia, for at least 6 hours, with a loss in recovery rate of about 10%. The data demonstrate a good potential of the new technique for measuring personal exposure to robust airborne viruses over a long period.