Contamination of aflatoxins in different kinds of foods in China.
Contamination of aflatoxins in different kinds of foods in China.
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发表时间：
2007-12
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影响因子：
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通讯作者：
Jun Wang;Xiu-mei Liu
中科院分区：
文献类型：
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作者：
Jun Wang;Xiu-mei Liu
目的了解我国6省2市玉米、花生、大米、核桃、松子等食品中总黄曲霉毒素的污染情况。方法随机抽取福建、广东、广西、湖北、江苏、浙江、上海、重庆等地市场销售的玉米、花生、大米、核桃、松子共283份样品。研磨样品，向其中加入乙腈/水溶液。过滤后，将提取物转移到MycoSep纯化柱中并缓慢加压。然后用三氟乙酸（TFA）衍生纯化的液体，并使用高效液相色谱（HPLC）测定。结果70.27%的玉米样品中检出AFs，平均含量为27.44 μ g/kg，最高含量为1098.36 μ g/kg。花生中AFs检出率为23.08%，平均水平为0.82 μ g/kg，最高水平为28.39 μ g/kg。只有极少数的大米样品被检测到有AF。核桃仁和松子仁中AFs含量很低。结论玉米是我国AFs污染最严重的食品。黄曲霉毒素B1是在食品中发现的主要黄曲霉毒素污染物。
OBJECTIVE To study the contamination of total aflatoxins (AFs) in different kinds of foods including corn, peanut, rice, walnut, and pine nut in six provinces and two municipalities in China. METHODS A total of 283 samples of corn, peanut, rice, walnut and pine nut were randomly collected from local markets in Fujian, Guangdong, Guangxi, Hubei, Jiangsu, and Zhejiang provinces, as well as in Shanghai and Chongqing municipalities. The samples were ground to which acetonitrile/water solution was added. After filtering, the extract was transferred into a MycoSep purifying column and was pressed slowly. Then the purified liquid was derivatized with trifluoroacetic acid (TFA) and assayed using high performance liquid chromatography (HPLC). RESULTS AFs were detected in 70.27% of corn samples, with a mean level of 27.44 microg/kg and the highest level of 1098.36 microg/kg. In peanut, the AFs detection rate was 23.08%, with a mean level of 0.82 microg/kg and the highest level of 28.39 microg/kg. Very few rice samples with AFs were detected. The AFs levels were very low in walnut and pine nut. CONCLUSION Corn is the food most seriously contaminated with AFs in China. AFB1 is the main aflatoxin which is found as a contaminant in foods.