Genomic structures of Cyp9m10 in pyrethroid resistant and susceptible strains of Culex quinquefasciatus
Genomic structures of Cyp9m10 in pyrethroid resistant and susceptible strains of Culex quinquefasciatus
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DOI：
10.1016/j.ibmb.2010.06.001
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发表时间：
2010-09-01
影响因子：
3.8
通讯作者：
Tomita, Takashi
中科院分区：
文献类型：
--
作者：
Itokawa, Kentaro;Komagata, Osamu;Tomita, Takashi
杀虫剂抗药性的发展降低了针对医学和农业害虫的控制措施的效力。细胞色素 P450 是参与杀虫剂代谢的主要解毒酶之一。此前，我们曾报道，与敏感菌株相比，P450 基因 Cyp9m10 在拟除虫菊酯抗性菌株中过度表达约 260 倍。在这项研究中，我们获得了直接证据表明 Cyp9m10 过度表达是由顺式作用突变引起的。此外，包括 Cyp9m10 基因座在内的长度约为 100 kb 的区域在抗性菌株中被特异性复制。两个复制的 Cyp9m10 拷贝在转录区域和侧翼区域（直到位于转录起始位点上游 1.1 kb 的断点）内共享完全相同的序列。将微型反向重复转座元件 (MITE) 样元件特意插入到抗性菌株中两个 Cyp9m10 拷贝上游 0.2 kb 处。在回交实验中，含有两个复制的 Cyp9m10 拷贝的单倍型与拟除虫菊酯抗性密切相关。 (C) 2010 Elsevier Ltd. 保留所有权利。
Development of insecticide resistance reduces the efficacy of controlling measures against the medical and agricultural insect pests. Cytochrome P450s are one of the major detoxification enzymes involved in insecticide metabolisms. Previously, we have reported that the P450 gene Cyp9m10 is about 260-fold overexpressed in a pyrethroid-resistant strain of Culex quinquefasciatus compared to a susceptible strain. In this study, we obtained direct evidence that the Cyp9m10 overexpression is caused by a cis-acting mutation. Additionally, a region of approximately 100 kb in length including the Cyp9m10 locus was specifically duplicated in the resistant strain. The two duplicated Cyp9m10 copies shared a completely identical sequence within the transcribed region and the flanking region up to the breakpoint located 1.1 kb upstream of the transcriptional start site. A Miniature Inverted-repeat Transposable Element (MITE)-like element was specifically inserted 0.2 kb upstream of both Cyp9m10 copies in the resistant strain. In backcross experiment, a haplotype containing the two duplicated Cyp9m10 copies was strongly associated with the pyrethroid resistance. (C) 2010 Elsevier Ltd. All rights reserved.