Atomic Force Microscopy Observation of Membrane Proteins Suspended over Carbon Nanotube Network
Atomic Force Microscopy Observation of Membrane Proteins Suspended over Carbon Nanotube Network
复制标题
原子力显微镜观察悬浮在碳纳米管网络上的膜蛋白
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
K. Torimitsu
中科院分区：
文献类型：
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作者：
K. Sumitomo;Youichi Shinozaki;D. Takagi;H. Nakashima;Yoshihiro Kobayashi;K. Torimitsu
原子力显微镜（AFM）对悬浮在纳米结构上的膜蛋白在液体中的成像是一种很有前途的了解工作蛋白结构和功能的方法，尽管在扫描过程中发生的膜变形使其难以获得高分辨率的图像。本研究提出了一种用于AFM观察功能性膜蛋白的人工细胞系统，该系统由硅上的亚微米孔、生物膜和碳纳米管（CNT）网络组成。我们成功地观察到悬浮在亚微米孔图案上的紫色膜的分子尺度图像。通过使用碳纳米管网络来固定悬浮膜，我们抑制了由“成像力”引起的膜变形。碳纳米管网络代替细胞骨架支撑悬浮在孔上的细胞膜，从而提高了AFM测量的空间分辨率。
Atomic force microscopy (AFM) imaging of membrane proteins suspended over a nanostructure in liquid is a promising way to understand the structure and function of working proteins, although the membrane deformation that occurs during scanning makes it difficult to obtain a high resolution image. This study proposes an artificial cell system for the AFM observation of functional membrane proteins that consists of a sub-micron well on Si, a biological membrane, and a carbon nanotube (CNT) network. We successfully observed molecular-scale images of a purple membrane suspended over sub-micron well patterns. By using a CNT network to hold the suspended membrane, we suppressed the membrane deformation caused by the “imaging force”. The CNT network takes the place of a cytoskeleton in supporting the cell membrane suspended over the well thus improving the spatial resolution of AFM measurement.