Conversion of Dorsal from an activator to a repressor by the global corepressor Groucho
Conversion of Dorsal from an activator to a repressor by the global corepressor Groucho
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DOI：
10.1101/gad.11.22.2952
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发表时间：
1997-11-15
影响因子：
10.5
通讯作者：
Courey, AJ
中科院分区：
文献类型：
--
作者：
Dubnicoff, T;Valentine, SA;Courey, AJ
在果蝇胚胎中，背部形态原既是转录的激活物，也是转录的抑制者，调节背部/腹侧花纹基因的表达。间接证据表明，背侧是一种内在的激活物，其他因素(辅助抑制物)将其转化为抑制物。然而，这些辅抑制子此前一直没有得到明确的鉴定。在这里，我们通过对缺乏母系编码的Groucho辅抑制子的胚胎的分析和蛋白质结合分析表明，通过蛋白质将Groucho招募到模板：蛋白质相互作用是将背部从激活物转换为抑制物所必需的。因此，Groucho是背侧/腹侧花纹系统的重要组成部分。
The Dorsal morphogen acts as both an activator and a repressor of transcription in the Drosophila embryo to regulate the expression of dorsal/ventral patterning genes. Circumstantial evidence has suggested that Dorsal is an intrinsic activator and that additional factors (corepressors) convert it into a repressor. These corepressors, however, have previously eluded definitive identification. We show here, via the analysis of embryos lacking the maternally encoded Groucho corepressor and via protein-binding assays, that recruitment of Groucho to the template by protein:protein interactions is required for the conversion of Dorsal from an activator to a repressor. Groucho is therefore a critical component of the dorsal/ventral patterning system.