Campylobacter jejuni-infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro-infected primary human intestinal tissue
Campylobacter jejuni-infected human epithelial cell lines vary in their ability to secrete interleukin-8 compared to in vitro-infected primary human intestinal tissue
复制标题
DOI：
10.1099/mic.0.29234-0
复制
发表时间：
2006-12-01
期刊：
影响因子：
2.8
通讯作者：
Everest, Paul H.
中科院分区：
文献类型：
--
作者：
MacCallum, Amanda J.;Harris, Dawn;Everest, Paul H.
空肠弯曲杆菌引起人类急性炎症性腹泻症状。空肠弯曲菌与肠上皮细胞相互作用，产生白介素8(IL-8)，白介素8将中性粒细胞招募到感染部位。细菌与上皮细胞相互作用的细胞培养模型对定义细菌与宿主的相互作用很有用，因为人们认为它们模仿了自然疾病中相同的细菌与宿主细胞的相互作用。这项研究着眼于空肠弯曲菌菌株从以前用于研究空肠弯曲菌与宿主细胞相互作用的各种细胞系中诱导IL-8产生的能力。观察到IL-8的反应谱，其中Caco-2细胞产生最低限度的IL-8，HeLa和T84细胞产生更显著的反应。将这些体外感染的细胞系的反应与体外感染空肠弯曲菌的人结肠和回肠组织产生的IL-8进行比较。体外感染的组织可以激发最高的IL-8反应，并且细胞因子比感染的细胞系出现得更早。
Campylobacter jejuni causes symptoms of acute inflammatory diarrhoea in man. C. jejuni interaction with epithelial cells elicits interleukin-8 (IL-8) production, and IL-8 recruits neutrophils to sites of infection. Cell culture models of bacterial interaction with epithelium are useful to define bacteria-host interaction and are used because it is thought they mimic the same bacteria-host cell interaction in the natural disease. This study looks at the ability of C. jejuni strains to elicit IL-8 production from a variety of cell lines previously used for investigating the interaction of C.jejuni with host cells. A spectrum of IL-8 responses was observed, with minimal IL-8 elicited from Caco-2 cells and more marked responses elicited from HeLa and T84 cells. These in vitro-infected cell line responses were compared to IL-8 production from in vitro C. jejuni-infected human colonic and ileal tissue. The in vitro-infected tissue elicited the highest IL-8 responses and the cytokine was manifested earlier compared to the infected cell lines.