An Experimental Test of Adaptive Introgression in Locally Adapted Populations of Splash Pool Copepods.
An Experimental Test of Adaptive Introgression in Locally Adapted Populations of Splash Pool Copepods.
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DOI：
10.1093/molbev/msaa289
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发表时间：
2021-04-13
影响因子：
10.7
通讯作者：
Kelly MW
中科院分区：
文献类型：
--
作者：
Griffiths JS;Kawji Y;Kelly MW
当物种努力跟上快速变暖的气候时，来自近亲物种或种群的有益等位基因的适应性渗入为快速适应提供了可能的途径。我们通过对两个耐热极限不同的种群进行杂交，研究了加利福尼亚虎脚类（Tigriopus californicus）的适应性渐渗的可能性。我们对杂交种进行了15代的强热选择，然后进行了全基因组重测序。利用杂交进化和重排序（HER）技术，我们可以通过等位基因频率的变化来识别响应热选择的位点。我们成功地提高了所选品系的耐热性（以LT50测量），这与来自南方（耐热）群体的高频率等位基因相结合。这些等位基因频率的可重复变化发生在所有独立选系的所有12条染色体上，提供了耐热性是多基因的证据。这些基因座所含的蛋白质编码序列差异低于全基因组平均水平，表明这些基因座在两个种群之间高度保守。此外，这些基因座富含在非致死热休克下改变选择系和对照系之间表达模式的基因。因此，我们假设耐热性分化的机制可以通过高度保守基因的差异基因表达来解释。HER方法为鉴定导致多基因性状的遗传变异提供了一种独特的解决方案，特别是通过其他群体基因组方法可能遗漏的变异。
As species struggle to keep pace with the rapidly warming climate, adaptive introgression of beneficial alleles from closely related species or populations provides a possible avenue for rapid adaptation. We investigate the potential for adaptive introgression in the copepod, Tigriopus californicus, by hybridizing two populations with divergent heat tolerance limits. We subjected hybrids to strong heat selection for 15 generations followed by whole-genome resequencing. Utilizing a hybridize evolve and resequence (HER) technique, we can identify loci responding to heat selection via a change in allele frequency. We successfully increased the heat tolerance (measured as LT50) in selected lines, which was coupled with higher frequencies of alleles from the southern (heat tolerant) population. These repeatable changes in allele frequencies occurred on all 12 chromosomes across all independent selected lines, providing evidence that heat tolerance is polygenic. These loci contained genes with lower protein-coding sequence divergence than the genome-wide average, indicating that these loci are highly conserved between the two populations. In addition, these loci were enriched in genes that changed expression patterns between selected and control lines in response to a nonlethal heat shock. Therefore, we hypothesize that the mechanism of heat tolerance divergence is explained by differential gene expression of highly conserved genes. The HER approach offers a unique solution to identifying genetic variants contributing to polygenic traits, especially variants that might be missed through other population genomic approaches.
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影响因子：
2.1
作者：
Ellison, C. K.;Burton, R. S.
通讯作者：
Burton, R. S.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.1
作者：
Kelly MW;DeBiasse MB;Villela VA;Roberts HL;Cecola CF
通讯作者：
Cecola CF
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
3.3
作者：
de Vladar, Harold P.;Barton, Nick
通讯作者：
Barton, Nick