Human peripheral blood eosinophils induce angiogenesis
Human peripheral blood eosinophils induce angiogenesis
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DOI：
10.1016/j.biocel.2004.09.001
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发表时间：
2005-03-01
影响因子：
4
通讯作者：
Levi-Schaffer, F
中科院分区：
文献类型：
--
作者：
Puxeddu, A;Alian, A;Levi-Schaffer, F
嗜酸性粒细胞在过敏反应和哮喘中起着至关重要的作用。它们还参与针对寄生虫、自身免疫和肿瘤疾病以及纤维化的反应。越来越多的证据表明血管生成在这些过程中发挥着重要作用。由于已知嗜酸性粒细胞产生血管生成介质，因此我们假设这些细胞对血管生成有直接贡献。研究了人外周血嗜酸性粒细胞超声处理对大鼠主动脉内皮细胞增殖（体外）、大鼠主动脉萌芽（离体）和鸡胚绒毛尿囊膜血管生成（体内）的影响。为了确定嗜酸性粒细胞衍生的血管内皮生长因子是否影响嗜酸性粒细胞促血管生成活性，将嗜酸性粒细胞超声处理物与抗血管内皮生长因子抗体一起孵育，然后添加到绒毛尿囊膜上。还评估了内皮细胞上的血管内皮生长因子mRNA表达和血管内皮生长因子受体密度。发现嗜酸性粒细胞可增强内皮细胞增殖，并在主动脉环和绒毛尿囊膜测定中诱导强烈的血管生成反应。嗜酸性粒细胞超声处理与抗血管内皮生长因子抗体的预孵育部分降低了绒毛尿囊膜中这些细胞的血管生成反应。嗜酸性粒细胞还增加内皮细胞上血管内皮生长因子 mRNA 的产生。嗜酸性粒细胞能够诱导血管生成，这种作用部分是由其预先形成的血管内皮生长因子介导的。这强烈表明嗜酸性粒细胞在血管生成相关疾病（例如哮喘）中发挥重要作用。 (C) 2004 Elsevier Ltd. 保留所有权利。
Eosinophils play a crucial role in allergic reactions and asthma. They are also involved in responses against parasites, in autoimmune and neoplastic diseases, and in fibroses. There is increasing evidence that angiogenesis plays an important role in these processes. Since eosinophils are known to produce angiogenic mediators, we have hypothesized a direct contribution of these cells to angiogenesis. The effect of human peripheral blood eosinophil sonicates on rat aortic endothelial cell proliferation (in vitro), rat aorta sprouting (ex vivo) and angiogenesis in the chick embryo chorioallantoic membrane (in vivo) have been investigated. To determine whether eosinophil-derived vascular endothelial growth factor influences the eosinophil proangiogenic activity, eosinophil sonicates were incubated with anti-vascular endothelial growth factor antibodies and then added to the chorioallantoic membrane. Vascular endothelial growth factor mRNA expression and vascular endothelial growth factor receptor density on the endothelial cells were also evaluated. Eosinophils were found to enhance endotheliat cell proliferation and to induce a strong angiogenic response both in the aorta rings and in the chorioallantoic membrane assays. Pre-incubation of eosinophil sonicates with anti-vascular endothelial growth factor antibodies partially reduced the angiogenic response of these cells in the chorioallantoic membrane. Eosinophils also increased vascular endothelial growth factor mRNA production on endothelial cells. Eosinophils are able to induce angiogenesis and this effect is partially mediated by their pre-formed vascular endothelial growth factor. This strongly suggests an important role of eosinophils in angiogenesis-associated diseases such as asthma. (C) 2004 Elsevier Ltd. All rights reserved.