Assessment of in Vitro Digestibility of Dietary Carbohydrates Using Rat Small Intestinal Extract
Assessment of in Vitro Digestibility of Dietary Carbohydrates Using Rat Small Intestinal Extract
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DOI：
10.1021/acs.jafc.7b01809
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发表时间：
2017-09-13
影响因子：
6.1
通讯作者：
Javier Moreno, F.
中科院分区：
文献类型：
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作者：
Ferreira-Lazarte, Alvaro;Olano, Agustin;Javier Moreno, F.
尽管非消化性碳水化合物在人类健康中的作用越来越重要，但对非消化性碳水化合物消化率的评估研究却很少。一系列可消化的膳食碳水化合物的体外消化率（麦芽糖，蔗糖和乳糖），公认的（乳果糖，低聚果糖（FOS）和两种主要糖苷键不同的低聚半乳糖（GOS）），以及潜在的（来自乳果糖的乳蔗糖和GOS，描述了在温度和pH的生理条件下使用大鼠小肠提取物（RSIE）制备OsLu）益生元的方法。公认的和潜在的益生元对RSIE消化具有高度抗性，尽管观察到不同程度的部分水解。FOS和乳果糖是最耐消化的，其次是OsLu，更远的是两种类型的GOS和低聚乳果糖。在GOS中，β（1 -> 6）键比β（1 -> 4）键更耐消化。报道的体外消化模型是一个有用的，简单的，和成本效益的工具来评估膳食低聚糖的消化率。
There are few studies on the assessment of digestibility of nondigestible carbohydrates, despite their increasingly important role in human health. In vitro digestibility of a range of dietary carbohydrates classified as digestible (maltose, sucrose, and lactose), well-recognized (lactulose, fructooligosaccharides (FOS), and two types of galactooligosaccharides (GOS) differing in the predominant glycosidic linkage), and potential (lactosucrose and GOS from lactulose, OsLu) prebiotics using a rat small intestinal extract (RSIE) under physiological conditions of temperature and pH is described. Recognized and potential prebiotics were highly resistant to RSIE digestion although partial hydrolysis at different extents was observed. FOS and lactulose were the most resistant to digestion, followed closely by OsLu and more distantly by both types of GOS and lactosucrose. In GOS, beta(1 -> 6) linkages were more resistant to digestion than beta(1 -> 4) bonds. The reported in vitro digestion model is a useful, simple, and cost-effective tool to evaluate the digestibility of dietary oligosaccharides.