Cytoplasmic acidification and the benzoate transcriptome in Bacillus subtilis.
Cytoplasmic acidification and the benzoate transcriptome in Bacillus subtilis.
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DOI：
10.1371/journal.pone.0008255
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发表时间：
2009-12-14
期刊：
影响因子：
3.7
通讯作者：
Slonczewski JL
中科院分区：
文献类型：
--
作者：
Kitko RD;Cleeton RL;Armentrout EI;Lee GE;Noguchi K;Berkmen MB;Jones BD;Slonczewski JL
枯草芽孢杆菌遇到了广泛的环境pH值。细菌保持细胞质pH值在一个狭窄的范围内。对酸胁迫的响应是外部pH和将质子传导到细胞质中的渗透酸的知之甚少的功能。在枯草芽孢杆菌中观察到细胞质酸化和苯甲酸盐转录组。使用GFPmut 3b荧光测定法以4-s时间分辨率测量细胞质pH。快速外部酸化（pH 7.5至6.0）酸化了B。枯草杆菌细胞质，然后部分回收。在外部pH 7下，加入高达60 mM的苯甲酸盐可降低细胞质pH，但留下了允许生长的跨膜ΔpH;这种对苯甲酸盐的强烈适应超过了在E.杆菌在用30 mM苯甲酸盐生长期间，细胞质pH值降低0.3个单位。通过比较在pH 7、+/-30 mM苯甲酸盐下生长后的4，095个基因表达指数来确定苯甲酸盐适应细胞的转录组。164个ORF显示苯甲酸盐（30 mM苯甲酸盐/0 mM）上调≥2倍，102个ORF显示下调≥2倍。42%的苯甲酸依赖性基因分别在pH 6和pH 7下上调或下调，它们是细胞质pH响应的候选者。苯甲酸酯上调的酸应激基因包括耐药性基因（yhbI、yhcA、yuxJ、ywoGH）、寡肽转运蛋白（opp）、甘氨酸催化酶（gcvPA-PB）、乙酸降解（阿克萨）、脱氢酶（ald、fdhD、serA、yrhEFG、yjgCD）、TCA循环（citZ、icd、mdh、sucD）和氧化应激（OYE家族yqjM、ohrB）。苯甲酸盐下调的碱胁迫基因包括苹果酸代谢（maeN），孢子形成控制（spo 0 M，spo 0 E）和SigW碱休克调节子。细胞质pH可介导碱休克诱导SigW。 B。枯草芽孢杆菌在生长过程中保持部分pH稳态，并承受高浓度的渗透酸胁迫，高于革兰氏阴性嗜中性菌E.杆菌苯甲酸适应转录组基本上重叠的外部酸，有助于细胞质pH转录组。
Bacillus subtilis encounters a wide range of environmental pH. The bacteria maintain cytoplasmic pH within a narrow range. Response to acid stress is a poorly understood function of external pH and of permeant acids that conduct protons into the cytoplasm. Cytoplasmic acidification and the benzoate transcriptome were observed in Bacillus subtilis. Cytoplasmic pH was measured with 4-s time resolution using GFPmut3b fluorimetry. Rapid external acidification (pH 7.5 to 6.0) acidified the B. subtilis cytoplasm, followed by partial recovery. Benzoate addition up to 60 mM at external pH 7 depressed cytoplasmic pH but left a transmembrane ΔpH permitting growth; this robust adaptation to benzoate exceeds that seen in E. coli. Cytoplasmic pH was depressed by 0.3 units during growth with 30 mM benzoate. The transcriptome of benzoate-adapted cells was determined by comparing 4,095 gene expression indices following growth at pH 7, +/− 30 mM benzoate. 164 ORFs showed ≥2-fold up-regulation by benzoate (30 mM benzoate/0 mM), and 102 ORFs showed ≥2-fold down-regulation. 42% of benzoate-dependent genes are regulated up or down, respectively, at pH 6 versus pH 7; they are candidates for cytoplasmic pH response. Acid-stress genes up-regulated by benzoate included drug resistance genes (yhbI, yhcA, yuxJ, ywoGH); an oligopeptide transporter (opp); glycine catabolism (gcvPA-PB); acetate degradation (acsA); dehydrogenases (ald, fdhD, serA, yrhEFG, yjgCD); the TCA cycle (citZ, icd, mdh, sucD); and oxidative stress (OYE-family yqjM, ohrB). Base-stress genes down-regulated by benzoate included malate metabolism (maeN), sporulation control (spo0M, spo0E), and the SigW alkali shock regulon. Cytoplasmic pH could mediate alkali-shock induction of SigW. B. subtilis maintains partial pH homeostasis during growth, and withstands high concentrations of permeant acid stress, higher than for gram-negative neutralophile E. coli. The benzoate adaptation transcriptome substantially overlaps that of external acid, contributing to a cytoplasmic pH transcriptome.
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影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S
DOI：
10.1016/j.ijfoodmicro.2008.09.015
发表时间：
2009-01-15
影响因子：
5.4
作者：
Chen, Jui-Lin;Chiang, Ming-Lun;Chou, Cheng-Chun
通讯作者：
Chou, Cheng-Chun
影响因子：
5.6
作者：
Cao, M;Kobel, PA;Helmann, JD
通讯作者：
Helmann, JD
影响因子：
3.6
作者：
DELL, CL;NEELY, MN;OLSON, ER
通讯作者：
OLSON, ER
影响因子：
4.2
作者：
Hayes ET;Wilks JC;Sanfilippo P;Yohannes E;Tate DP;Jones BD;Radmacher MD;BonDurant SS;Slonczewski JL
通讯作者：
Slonczewski JL