Hydroxyapatite-chitin materials as potential tissue engineered bone substitutes
Hydroxyapatite-chitin materials as potential tissue engineered bone substitutes
复制标题
DOI：
10.1016/s0142-9612(03)00612-4
复制
发表时间：
2004-03-01
期刊：
影响因子：
14
通讯作者：
Khor, E
中科院分区：
文献类型：
--
作者：
Ge, ZG;Baguenard, S;Khor, E
将25%、50%和75% w/w的羟基磷灰石（HA）加入几丁质溶液中，加工成空气和冻干材料。这些ha -几丁质材料暴露于细胞培养并植入大鼠模型的肌内。发现ha -几丁质材料在体内无细胞毒性且可降解。透明质酸填料的存在增强了钙化，并加速了甲壳素基质的降解。冻干的ha -甲壳素基质由于其多孔性而被选择用于进一步的细胞播种实验。利用地塞米松体外诱导NZW兔间充质干细胞成骨。将这些成骨细胞静载于多孔ha -几丁质基质上，植入兔股骨骨缺损2个月。外植体的组织学表现为ha -几丁质基质的生物降解再生。同样，绿色荧光蛋白（GFP）转染的msc诱导的成骨细胞也被装载到多孔ha -几丁质基质上并植入兔子股骨。gfp转染MSCs的结果表明，装载MSCs诱导的成骨细胞不仅增殖，而且还招募周围组织生长。本研究证明了ha -几丁质基质作为组织工程骨替代物的良好候选基质的潜力。(C) 2003 Elsevier Ltd.版权所有。
Hydroxyapatite (HA) in 25%, 50% and 75% w/w fractions was incorporated into chitin solutions and processed into air- and freeze-dried materials. These HA-chitin materials were exposed to cell cultures and implanted into the intramusculature of a rat model. The HA-chitin materials were found to be non-cytotoxic and degraded in vivo. The presence of the HA filler enhanced calcification as well as accelerated degradation of the chitin matrix. The freeze-dried HA-chitin matrixes were selected for further cell seeding experiments because of their porous nature. Mesenchymal stem cells harvested from NZW rabbits were induced into osteoblasts in vitro using dexamethasone. These osteoblasts were cultured for 1 week, statically loaded onto the porous HA-chitin matrixes and implanted into bone defects of the rabbit femur for 2 months. Histology of explants showed bone regeneration with biodegradation of the HA-chitin matrix. Similarly, green fluorescence protein (GFP) transfected MSC-induced osteoblasts were also loaded onto porous HA-chitin matrixes and implanted into the rabbit femur. The results from GFP-transfected MSCs showed that loaded MSCs-induced osteoblasts did not only proliferate but also recruited surrounding tissue to grow in. This study demonstrates the potential of HA-chitin matrixes as a good substrate candidate for tissue engineered bone substitute. (C) 2003 Elsevier Ltd. All rights reserved.