An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion.
An important role of phospholipase Cgamma1 in pre-B-cell development and allelic exclusion.
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磷脂酶 Cgamma1 在前 B 细胞发育和等位基因排除中的重要作用。
DOI：
10.1038/sj.emboj.7600405
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Wang,Demin
中科院分区：
文献类型：
--
作者：
Wen,Renren;Chen,Yuhong;Schuman,James;Fu,Guoping;Yang,Shoua;Zhang,Weiguo;Newman,DebraK;Wang,Demin
Phospholipase Cγ1 (PLCγ1) has been reported to be expressed predominantly in T cells and to play an important role in T‐cell receptor signaling. Here we show that PLCγ1 is expressed throughout B‐cell development, with high expression in B‐cell progenitors, and is involved in pre‐B‐cell receptor (pre‐BCR) signaling. Reduced expression of PLCγ1, in the absence of PLCγ2 (PLCγ1+/−PLCγ2−/−), impedes early B‐cell development at the pro‐B‐ to pre‐B‐cell transition and impairs immunoglobulin heavy chain allelic exclusion, hallmarks of defective pre‐BCR signaling. In contrast, early B‐cell development is largely normal, whereas late B‐cell maturation is impaired in the absence of PLCγ2 alone (PLCγ2−/−) and overexpression of PLCγ1 in PLCγ2−/−mice fails to restore BCR‐mediated B‐cell proliferation and maturation. These studies reveal an essential role of PLCγ1, distinct from that of PLCγ2, in B‐cell development.
