Prolonged bursts occur in normal calcium in hippocampal slices after raising excitability and blocking synaptic transmission.
Prolonged bursts occur in normal calcium in hippocampal slices after raising excitability and blocking synaptic transmission.
复制标题
在提高兴奋性和阻断突触传递后，海马切片中的正常钙会出现长时间的爆发。
DOI：
10.1152/jn.2001.86.5.2625
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Stringer,JL
中科院分区：
文献类型：
--
作者：
Xiong,ZQ;Stringer,JL
这项研究检查了在海马体切片中记录的长时间癫痫样活动出现所需的条件。在正常钙水平下，用高钾、铯、4-氨基吡啶或四乙基氯化铵灌注海马切片，可诱导CA1和CA3自发性间期活动。然后通过添加神经递质受体阻滞剂（6-氰基-7-硝基喹啉-2,3-二酮，d，l-2-氨基-5-磷酸戊酸和双管碱）或钙通道阻滞剂镉阻断突触传递，导致完全阻断间期放电和主要在CA1和齿状回出现自发性癫痫样事件（癫痫样放电）。在正常人工脑脊液中阻断突触传递不会引起任何区域的癫痫样放电。结果表明，当化学突触传递被药物阻断时，在正常水平的细胞外钙离子中可出现ictal样放电。结果表明，神经元兴奋性的增加和间歇活动的缺乏促进了更长时间的类似于发作的放电的出现。
This study examined the conditions that are required for the appearance of the long-duration seizure-like activity that can be recorded in hippocampal slices. Spontaneous interictal activity was induced in CA1 and CA3 by perfusing hippocampal slices with high potassium, cesium, 4-aminopyridine, or tetraethylammonium chloride, in normal levels of calcium. Synaptic transmission was then blocked by the addition of neurotransmitter receptor blockers (6-cyano-7-nitroquinoxaline-2,3-dione,d,l-2-amino-5-phosphonopentanoic acid, and bicuculline) or the calcium channel blocker cadmium, resulting in complete blockade of the interictal discharges and the appearance of spontaneous seizure-like events (ictal-like discharges) primarily in CA1 and the dentate gyrus. Blocking synaptic transmission in normal artificial cerebrospinal fluid did not induce ictal-like discharges in any region. The results demonstrate that ictal-like discharges can appear in normal levels of extracellular calcium when chemical synaptic transmission is blocked pharmacologically. The results suggest that an increase in neuronal excitability and absence of interictal activity promote the appearance of the longer ictal-like discharges.