LIFE-CYCLE OF NEMATODE CAENORHABDITIS-ELEGANS .1. WILD-TYPE GROWTH AND REPRODUCTION
LIFE-CYCLE OF NEMATODE CAENORHABDITIS-ELEGANS .1. WILD-TYPE GROWTH AND REPRODUCTION
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DOI：
10.1016/0012-1606(76)90119-6
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发表时间：
1976-01-01
影响因子：
2.7
通讯作者：
RUSSELL, RL
中科院分区：
文献类型：
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作者：
BYERLY, L;CASSADA, RC;RUSSELL, RL
使用电子线虫计数器研究了小型线虫线虫的生长和繁殖。 20度时。 C，通常的生长温度，大小随时间以平滑的 S 形方式增加，线性生长在第四次蜕皮时最为迅速，并在产卵期结束时几乎停止。人口增长高度同步；小残留尺寸异质性在大约最大生长时达到最大。 4 次蜕皮并不涉及生长的重大中断，但确实会导致轻微的形状变化（脱离旧角质层后伸长）。第四次蜕皮后不久就开始产卵，第一次蜕皮速度迅速上升，然后逐渐达到峰值，随后相对快速下降。 16 度和 25 度下的可比测量值C 确定这些对于温度敏感突变体来说是可接受的极限温度，尽管在 25°C 下产蛋率有所降低并且产蛋动力学发生改变。 C. 列出了所有 3 个温度的发育年表。
The growth and reproduction of the small nematode C. elegans was studied using an electronic nematode counter. At 20.degree. C, the usual growth temperature, size increases in a smooth sigmoidal manner with time, linear growth being most rapid around the time of the 4th molt and nearly ceasing by the end of the period of egg-laying. Growth of populations is highly synchronous; the small residual size heterogeneity is maximal at about the time of maximal growth. The 4 molts do not involve major interruption of growth, but they do entail slight shape changes (elongating upon escape from the old cuticle). Egg-laying begins shortly after the 4 molt, the rate rising rapidly at 1st, then more gradually to a peak followed by a relatively rapid fall. Comparable measurements at 16 and 25.degree. C establish that these are acceptable limit temperatures for work with temperature-sensitive mutants, although egg yield is somewhat reduced and the kinetics of egg-laying are altered at 25.degree. C. Developmental chronologies for all 3 temperatures are presented.