MACROPHAGE DEPLETION IN THE RAT AFTER INTRAPERITONEAL ADMINISTRATION OF LIPOSOME-ENCAPSULATED CLODRONATE - DEPLETION KINETICS AND ACCELERATED REPOPULATION OF PERITONEAL AND OMENTAL MACROPHAGES BY ADMINISTRATION OF FREUNDS-ADJUVANT
MACROPHAGE DEPLETION IN THE RAT AFTER INTRAPERITONEAL ADMINISTRATION OF LIPOSOME-ENCAPSULATED CLODRONATE - DEPLETION KINETICS AND ACCELERATED REPOPULATION OF PERITONEAL AND OMENTAL MACROPHAGES BY ADMINISTRATION OF FREUNDS-ADJUVANT
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DOI：
10.1007/bf00304524
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发表时间：
1995-04-01
影响因子：
3.6
通讯作者：
VANROOIJEN, N
中科院分区：
文献类型：
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作者：
BIEWENGA, J;VANDERENDE, MB;VANROOIJEN, N
本研究的目的是建立一种从大鼠腹腔和网膜中清除巨噬细胞的方法。大鼠接受两次腹膜内注射（在第0天和第3天）脂质体包封的氯膦酸盐（二氯亚甲基二膦酸盐：Cl（2）MBP-脂质体）。该处理导致成熟组织巨噬细胞（ED 2阳性巨噬细胞）在2天内从腹腔和网膜中完全消除。消除包括网膜的强ED 2阳性梭形细胞。在接下来的23天内未观察到网膜ED 2阳性巨噬细胞的再增殖。而ED 2阳性巨噬细胞完全耗尽，ED 1阳性细胞很少，ED 1阳性细胞的再增殖更快。该处理进一步消耗了来自脾脏的巨噬细胞，特别是来自红髓、甲状旁腺淋巴结和肝脏的巨噬细胞。在最后一次注射Cl（2）MBP-脂质体后一天给予弗氏不完全佐剂，大大加速了网膜中的再增殖。所描述的协议可用于调查的贡献成熟组织巨噬细胞诱导免疫反应，药物代谢和消除肠道肿瘤。
The purpose of this study was to develop a method for the depletion of macrophages from the peritoneal cavity and the omentum of the rat. Rats received two intraperitoneal injections (at days 0 and 3) with liposome-encapsulated clodronate (dichloromethylene bisphosphonate: Cl(2)MBP-liposomes). This treatment resulted in complete elimination of mature tissue macrophages (ED2-positive macrophages) from the peritoneal cavity and the omentum within 2 days. The elimination included the strongly ED2-positive spindle-shaped cells of the omental membrane. Repopulation of the omental ED2-positive macrophages was not seen within the next 23 days. Whereas ED2-positive macrophages were completely depleted, few ED1-positive cells remained and repopulation of ED1-positive cells was faster. The treatment further depleted macrophages from the spleen, especially from the red pulp, parathymic lymph nodes and liver. Freund's incomplete adjuvant administered one day after the last injection of Cl(2)MBP-liposomes considerably accelerated repopulation in the omentum. The protocol described might be used to investigate the contribution of mature tissue macrophages to the induction of immune responses, drug metabolism and the elimination of intestinal tumours.