A computer controlled analysis of freezing behaviour
A computer controlled analysis of freezing behaviour
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DOI：
10.1016/s0165-0270(98)00150-2
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发表时间：
1998-12-31
影响因子：
3
通讯作者：
Feldon, J
中科院分区：
文献类型：
--
作者：
Richmond, MA;Murphy, CA;Feldon, J
心理学家和神经科学家经常使用大鼠的条件性冻结反应来研究大脑损伤和动机状态变化对行为的影响。冻结反应的主要优点是，它可以在没有诸如食物或水剥夺等动机操纵的情况下使用。在此之前，冻结已经由人类观察者通过视频记录或在测试过程中测量。但这些数据收集方法有潜在的缺点。在本文中，我们描述了一种新的，计算机控制的，自动化的程序来评估条件冻结。每个调节室包含一个微型摄像机。行为是通过一个程序进行在线分析的，该程序每隔两秒钟比较一次录像带，产生一个屏幕，显示它们之间的百分比差异。< 0.05%（50像素）的差异被归类为冻结响应。实验中，我们测量条件冻结及其发展随着时间的推移，在响应上下文线索和离散的音调已配对与脚电击。我们证明了我们的设备和方法的数据分析是敏感的：音调休克配对，大鼠应变和音调预曝光的数量。(C)1998 Elsevier Science B.V.保留所有权利。
The conditioned freezing response in rats has been much used both by psychologists and neuroscientists to investigate the behavioural effects of brain lesions and of changes in motivational state. The primary advantage of the freezing response is that it can be used without motivational manipulations such as food or water deprivation. Previously, freezing has been measured by a human observer either from video recordings or during the test sessions themselves. But these methods of data collection have potential disadvantages. In the present paper we describe a new, computer controlled, automated procedure for assessing conditioned freezing. Each conditioning chamber contains a mini-video camera. Behaviour is analysed on-line by means of a programme which compares every two adjacent seconds of video tape to generate a screen representing the percentage difference between them. A difference of < 0.05% (50 pixels) is classified as a freezing response. Experiments are described in which we measure conditioned freezing and its development over time, in response to contextual cues and to a discrete tone which had been paired with foot shock. We demonstrate our apparatus and methods of data analysis to be sensitive to: number of tone-shock pairings, rat strain and tone pre-exposure. (C) 1998 Elsevier Science B.V. All rights reserved.