Immunoprofiling reveals unique cell-specific patterns of wall epitopes in the expanding Arabidopsis stem
Immunoprofiling reveals unique cell-specific patterns of wall epitopes in the expanding Arabidopsis stem
复制标题
DOI：
10.1111/tpj.12111
复制
发表时间：
2013-04-01
期刊：
影响因子：
7.2
通讯作者：
Ellis, Brian E.
中科院分区：
文献类型：
--
作者：
Hall, Hardy C.;Cheung, Jingling;Ellis, Brian E.
拟南芥花序茎经历快速定向生长，需要大量的轴向细胞壁延伸在其所有组织中，但是，在成熟时，这些组织由表现出明显不同的细胞壁结构的细胞类型组成。目前尚不清楚这些细胞类型的细胞壁成分是否在轴向生长停止后迅速发散，或者成分发散是否发生在分化的早期阶段，尽管细胞壁的可伸展性是共同的要求。为了研究这个问题，7种细胞类型进行了测定的丰度和分布的18个主要细胞壁聚糖类在三个发展阶段沿着发展的花序干，使用高通量免疫标记策略。这些阶段代表了幼年生长的阶段，显示茎延伸的最大速率的阶段，以及延伸生长停止的阶段。检测到的免疫标记模式表明，大多数干组织的细胞壁组成经历了显着的变化过程中和快速延伸生长后。免疫标记信号的分层聚类鉴定了一些抗体的细胞特异性结合模式，包括阿拉伯半乳聚糖侧链定向抗体的亚组，其表位靶点在快速细胞扩增阶段期间与束间纤维区特异性相关。这些数据揭示了拟南芥花序干细胞壁扩张和成熟过程中不同细胞类型细胞壁化学的动态，细胞类型特异性变化，并突出了这种结构多样性和干生长过程中所有组织发生的均匀各向异性细胞扩张之间的矛盾。
The Arabidopsis inflorescence stem undergoes rapid directional growth, requiring massive axial cell-wall extension in all its tissues, but, at maturity, these tissues are composed of cell types that exhibit markedly different cell-wall structures. It is not clear whether the cell-wall compositions of these cell types diverge rapidly following axial growth cessation, or whether compositional divergence occurs at earlier stages in differentiation, despite the common requirement for cell-wall extensibility. To examine this question, seven cell types were assayed for the abundance and distribution of 18 major cell-wall glycan classes at three developmental stages along the developing inflorescence stem, using a high-throughput immunolabelling strategy. These stages represent a phase of juvenile growth, a phase displaying the maximum rate of stem extension, and a phase in which extension growth is ceasing. The immunolabelling patterns detected demonstrate that the cell-wall composition of most stem tissues undergoes pronounced changes both during and after rapid extension growth. Hierarchical clustering of the immunolabelling signals identified cell-specific binding patterns for some antibodies, including a sub-group of arabinogalactan side chain-directed antibodies whose epitope targets are specifically associated with the inter-fascicular fibre region during the rapid cell expansion phase. The data reveal dynamic, cell type-specific changes in cell-wall chemistry across diverse cell types during cell-wall expansion and maturation in the Arabidopsis inflorescence stem, and highlight the paradox between this structural diversity and the uniform anisotropic cell expansion taking place across all tissues during stem growth.